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same precautions as before, and hung up under a bell jar by 
wires which had been heated and cooled. The liquid in the 
eprouvette @ was subjected to repeated examination until 
March 3, when it was still perfectly limpid and entirely free 
from organic forms. A single drop of common distilled water 
was then added to it. Ina few days it became milky and 
acquired a putrid smell. In the glass 4, there were already 
signs of bacteria on March 2, and the liquid soon became 
offensive. The thighs which were hung up, shortly became 
covered with a crust of penicillium. One of them was ex- 
amined on March 9. On removing the crust and cutting 
into the muscle it was found to be less moist, but otherwise 
of natural appearance. There was a musty but no putre- 
factive smell. The cut surface was neutral to test paper. 
The other thigh was examined March 27, and was in a 
similar condition, excepting that the muscular substance was 
drier and of darker colour. 
XXI1iI.—February 1.—Five centimetres of urine were 
introduced into an eprouvette, which was then plugged with 
cotton wool and placed under a glass shade. It retained its 
acid reaction and limpidity till February 9, when a drop or 
two of ordinary cold distilled water was introduced from a 
fine capillary pipette prepared just before. On the 16th the 
liquid was hazy and crowded with bacteria. In the course 
of a few days more, a sediment subsided to the bottom of 
the eprouvette, and the liquid became alkaline and ammoni- 
acal. This experiment was subsequently repeated with similar 
results. 
It has been long known that the tendency of urine to 
undergo decomposition may be obviated by protecting it 
against contamination from without. The preceding experi- 
ments show that here, as elsewhere, water is the contami- 
nating agent. 
XXIV.—January 2.—An abundant flow of saliva having 
been determined by introducing a few drops of ether into 
the mouth, one or two drops were allowed to fall into a 
charged eprouvette. The liquid was repeatedly examined 
during the next three weeks, but no microzymes could be 
detected. The salivary secretion, as it is discharged from 
the salivary ducts, is no doubt inactive; but inasmuch as the 
mixed liquid with which the mucous membrane is moistened 
is exposed to several sources of contamination, and moreover 
can be often shown to contain leptothrix filaments, it would 
not have been surprising if the result of the experiment had 
been otherwise. 
XXV.—It is scarcely possible to obtain milk in a state of 
