398 
carry the “ cultivation ” in this species further whilst on the 
object-slide. However, in C. spinosa, luckily it was possible 
to get astep further. In some vigorous specimens which I 
caught at Naples in February, nearly all the Capsules were 
filled by an immense number of small spheroids (fig. 7). 
These Collosphera colonies were laid in large flat vessels 
filled with sea-water, and in order to prevent the water 
becoming bad, bits of ulva and other green Algz were placed 
in with them. After one day I found, instead of the common 
sausage-shaped or spherical colonies which I placed in the 
vessel, masses of yellow granules which when taken out with 
a glass tube and examined under the microscope proved to be 
Capsules of C. spinosa. ‘The alveoli to which they had been 
adherent were entirely gone, only a trace remained here and 
there of the radial protoplasm, sticking on to the Capsules. 
The Capsules were thickly squeezed together. At the first 
glance the specimens seemed in the act of dying, and I was 
just going to throw them away, when I observed in several 
Capsules a tremulous movement of the enclosed corpuscles, 
which in a short time manifested itself in nearly all the 
Capsules of the mass, and ended with a copious outpouring 
or “‘ swarming.” 
I could now quite comfortably observe a part of the ma- 
terial with the higher powers under a covering glass (care 
being taken to prevent its pressing too heavily) as well as in 
hanging drops.! 
In nearly every Capsule monad-like organisms vibrated, 
the liberated ones swam meanwhile actively in all directions 
round about. By the side of capsules, whose contents still 
remained homogeneous, not differentiated, lay those which 
were full of as yet quiescent, others full of moving, corpuscles. 
From one capsule I saw the latter issue forth in mass at one 
point (fig. 8). In some cases I believe I have quite clearly 
observed how they passed through the fenestre of the shell. 
Let us now pay somewhat closer attention to the little bodies — 
swimming here and there around, which I shall henceforth 
speak of as zoospores. The Collosphera zoospores are ‘(008 mm. 
long, oval, somewhat obliquely trimmed away at the smaller 
end, which carries two long cilia (figs. 9,10). In all the 
zoospores I found a crystalline little rod, ‘004 mm. in length, 
rounded at either end, or brought to a point, which often 
projected out somewhat from the body. Add a few oil drops, 
and you have almost all that one can make out of form- 
elements in the naked protoplasmic bodies of the zoospores. 
1 Prof. Cienkowski alludes to the use of a chamber such as that described 
in my paper in this number of the Journal.—E. R, L, 
