282 NOTES AND MEMORANDA. 



Submerge the tissue to be stained, in the necessary quan- 

 tity of an ordinary carmine-staining fluid, such as either 

 Beale's or Rutherford's fluid, until it has become of a de- 

 cided carmine tint ; next drop into this fluid containing the 

 preparation a little of Draper's ^ dichroic ink in the propor- 

 tion of about four drops of the ink to each drachm of the 

 carmine stain. Shake the containing vessel gently until the; 

 two fluids are thoroughly mixed. 



The length of time the preparation should remain in this 

 mixed fluid will depend upon its thickness, and will vary 

 from six to twenty-four or to forty-eight hours. 



When sufiiciently coloured, remove the preparation from the 

 fluid and wash carefully with either filtered or distilled water 

 until it ceases to impart a tint to the water. It is then ready 

 for mounting in Price's glycerine. 



I believe that logwood is one of the ingredients of the ink, 

 the true composition of which is a trade secret. Possibly 

 ordinary logwood stain would answer the purpose as well as 

 the ink, but of this I have no experience, having found the 

 ink so eflicacious. 



I have some very beautiful preparations stained in the 

 manner above mentioned and mounted in glycerine ; of these 

 organic muscle and delicate fasciae are probably the most 

 striking. — B. Wills Richardson, F.R. C.S.I. 



Dr. Reyher on Synovial Membranes. — The memoir promised 

 in our last number has been withdrawn by the author (whose 

 name was printed by an error as Reijner), with a view to its 

 earlier publication in Germany and this country. 



Action of Fresh Cholera-Ejections upon Animals. — Hogyes 



(^Centralhlatt fur die Meclicinischen Wissenschaften, Nos. 

 50 and 51) states the results of his experiments on the action of 

 fresh cholera-ejections upon dogs and rabbits. The injections 

 were employed one and one and a half hours after passing from 

 the patient. The author has found — 1. That fresh cholera-ejec- 

 tions act injuriously upon the animal organism, and, as it 

 seems, on diflerent animals in diflerent degrees. 2. The chief, 

 or at least most constant, phenomenon of the injurious action 

 after every form of introduction of the cholera-injections, is a 

 more or less strongly pronounced inflammatory change of the 

 stomach and intestinal tract. 3. A catarrh of the stomach 

 or intestine artificially produced renders the animal more 

 susceptible of the injurious efiect. 4. Inspiration of air 

 saturated with particles from non- disinfected cholera-ejec- 



' Mary Street, Dublin. 



