156 DR. E. KLEINi 



V. Ebner, but show a very beautiful and dense network of fibrils ; 

 hence their apparent granulation. Preparations of the " papilla 

 foliata" of rabbit's tongue hardened first with Muller's fluid and 

 then with methylated alcohol, as also the parts of human tongue 

 containing the circumvallate papillae hardened in our mixture 

 of chromic acid and methyl, alcohol above mentioned, yield very 

 good results. AH that is necessary is to examine a very thin 

 part of a section, stained with logwood, with a good high 

 power. It will then be also noticed that the nucleus does 

 not contain a " granular " substance nor a nucleolus, but a 

 uniform network with the usual dots in the nodes. The intranu- 

 clear fibrils can be traced through the membrane of the nucleus 

 into the intracellular network. At the same time we notice 

 that whereas the cells lining the peripheral alveoli of the lobules 

 in the serous glands of rabbit are polygonal as stated by v. 

 Ebner, they are beautiful columnar cells in the glands of man. 



5 . The Cells of the Glands of the Stomach and the Duodenum. 



In this paragraph I wish merely to state my observations as 

 regards the structure of the cells lining the above glands. 



(a) The glands of the fundus. — As is well known from the 

 researches of Heidenhain,i Eollett" and others, the cells called by 

 Heidenhain chief cells, by Rollett adelomorphous cells, are distin- 

 guished from the parietal cells (Heidenhain), or delomorphous 

 cells (Rollett) , not only by their different shape and position, but 

 also by their different aspect, the former being more transparent 

 than the latter, which by their " granulation" are always more 

 conspicuous. Examining the two kinds of cells in the glands of 

 the fundus of man, pig, cat and dog, in the fresh state,^or after 

 maceration, or in specimens hardened with chromic acid and 

 spirit, I find that the substance of both the chief cells and 

 parietal cells is composed of a more or less distinct network of 

 fibrils with this difference, that in the latter the network is much 

 denser, hence its " granular'^ appearance. And also the nuclei 

 of both show a uniform network, especially the nucleus of the 

 parietal cells is, in this respect, of a very regular appearance . 

 The best method to show these minute structures is to harden 

 the above glands, especially of dog, first in a mixture of chromic 

 acid (-2 parts of ^ per cent.), and methylated alcohol (1 part), 

 and after four or five days to complete the hardening in methv- 

 lated spirit. Thin sections stained in hsematoxylin show 



1 ' Archiv f. Mikrosk. Anat., Band, vi, p. 336 



2 ' Ccntralblaf.t,' 1S70, Nos. 21 and 22; and Rollett's 'Uutersuchun"-en ' 

 2 Heft., 1S71. *utuun„en, 



