386 



TIMOTHY RICHARDS LEWIS. 



characters of the blood by perusing an account, in the ' Veteri- 

 nary Journal/ of " worms" having been found in the blood of 



Fig. 14.— Orsjanisms found in the tissues of healthy animals a few hours 

 after death, x 1500 diam. 



horses suffering from a similar affection in the Punjab. A slide 

 was prepared and examined under the microscope at once, but no 

 marked peculiarity could be detected ; but when this and other 

 slides were re-examined twelve hours later, having in the mean- 

 while been kept under a bell glass, numerous staves and filaments 

 were observed, which, as to size and form, accurately corre- 

 sponded with the description of like bodies characterising the 

 blood in anthracoid diseases in Europe, 



Several " cultivations" were started by adding a little of the 

 blood to fresh aqueous humour. The preparations were then set 

 aside for a few hours in a moist chamber. As the temperature 

 of the atmosphere at that time was generally over 90° T., no 

 special appliances were necessary for supplying artificial heat. 

 The development of the rods into filaments and subsequent 

 appearance of highly refractive oval bodies in the latter corre- 

 sponded so completely with what Cohn, Koch, Ewart, and others 

 have described, that it is not necessary to give figures of the 

 changes that took place. A series of such cultivations was 

 conducted by transferring a little of the last cultivation to fresh 

 aqueous humour, and so on from one preparation to another. 



It was then determined to ascertain whether the bacilli found 

 in the blood of animals which had been set aside for a few hours 

 after death would manifest, under like conditions, similar changes 

 during, their growth. Rats were obtained, killed by means of 

 chloroform, and set aside for from three to twenty-four hours, or 

 longer according as the temperature of the atmosphere was high 

 or low. The result proved that, almost invariably, bacilli were 



