CHLOROPHYLL-CORPUSCLES AND AMYLOID DEPOSITS. 245 



tlian one such nucleus was observed (2 to 6), which were 

 regarded as indications of a process of division. 



(4) The green corpuscles were maintained on the object 

 slide after isolation from the surrounding cell-protoplasm, 

 and were observed to retain their form for several days or 

 even weeks. When exposed to the light such green cor- 

 puscles (from Spongilla as well as from Hydra) develop 

 starch-grains within their substance. 



(5) Isolated chlorophyll-corpuscles from Spongilla were 

 brought into association with ciliated Infusoria, which 

 swallowed the chlorophyll-corpuscles. The corpuscles were 

 either digested or ejected unchanged. 



On the other hand, the larger chlorophyll-corpuscles of 

 Hydra viridis, when similarly swallowed, were found to 

 remain unchanged in the Infusoria for a certain time. Dr. 

 Brandt does not state that these latter corpuscles multiplied 

 by division in the body of the Infusorian which had swal- 

 lowed them. 



Upon the grounds summarised in these five paragraphs, 

 Dr. Brandt concludes that chlorophyll is never formed by 

 animal organisms, but when found in animal cells is due to 

 the presence of parasitic Algse, to which he has given generic 

 and specific names. 



It seems to me that even if we accept every word of Dr. 

 Brandt^s statement as to the structure of the chlorophyll- 

 corpuscles there is not sufficient ground for adopting his 

 conclusions. With regard to the statement contained in 

 paragi-aph 2, 1 am in agreement with Dr. Brandt. 



As to the existence of a cell-nucleus (paragraph 3) in the 

 chlorophyll-corpuscles either of Spongilla or Hydra, I am 

 at variance with him. I have not used hsematoxylin as a 

 staining agent in this inquiry, but picro-carmine, and I had 

 very fully satisfied myself that nothing like a cell-nucleus 

 exists in connection with the green corpuscles in either 

 Spongilla or Hydra previously to Dr. Brandt's statements. 

 I have examined them in various ways, including that of 

 removing the green pigment before treatment with picro- 

 carmine. I have found that when a slight staining only is 

 used, sufficient to colour well the nucleus of the amoeboid 

 sponge-cell or of the endoderm-cell of Hydra, no coloration 

 of the protoplasm in connection with the chlorophyll-cor- 

 puscles is to be seen ; but if a strong staining be allowed to 

 take place, then the protoplasm of the general substance of 

 the cell becomes pink as well as the nucleus of the cell, but 

 in a less degree; and if the chlorophyll-corpuscles be 

 squeezed out of cells of Spongilla so treated, then the little 



