114 WYMAN, ON THE FORMATION OF INFUSORIA. 
of the water. The flask was opened on the fifth day, and 
found to contain Vibrios, Bacteriums, and a few ferment cells. 
The gelatinous exudation on the surface of the meat also 
contained the same organisms, and appeared to be wholly 
made up of them. 
Exp. 12. (2) March 13th. The juice of an ounce of beef, 
to which was added 10 c.c. of urme and 40 c.c. of water was 
boiled twenty minutes in a bolt-head and hermetically sealed. 
A film formed on the fourth, and the flask was opened on the 
eleventh day, when there was a distinct rush of air outwards. 
Large numbers of Bacteriums were found, also small spheri- 
cal bodies with ciliary motions and oval bodies like Kolpoda, 
containing what appeared to be Bacteriums; one of these 
Kolpoda-like bodies moved with cilia. 
Exp. 13. (1) March 15th. An ounce of beef was suspended 
over water in a flask of 500 c.c. capacity and boiled twenty- 
five minutes. The same changes took place as in the pre- 
ceding experiment. The exudation appeared on the surface 
of the meat, and the film on the water on the second day ; 
on the fourth day the meat fell to pieces; the flask was 
opened on the ninth day; Bacteriums were found in large 
numbers, and there was a slight odour of putrefaction. 
Exp. 14. (1) March 17th. Juice of beef and a few shreds 
of beef were boiled in a flask of 300 c.c. capacity for twenty 
seconds. A film was formed on the second day, and the flask 
was opened on the eleventh day. Bacteriums existed in 
abundance. 
Exp. 15. (1) March 19th. Fifty cubic centimetres of beef 
were boiled forty minutes in a flask of 800 c.c. capacity. 
The film began to form on the second day, and the flask was 
opened on the 24th. The film had disappeared, the fluid had 
a nauseous odour, and the Bacteriums were diffused through 
the whole mass instead of being mostly confined to the sur- 
face as in the preceding experiments. 
Expts. 16, 17, 18, 19. (2) March 20th. Were made with 
juice of beef and water in flasks of 550 c.c. capacity ; 16 was 
boiled fifteen minutes, the film formed on the second day, and 
the flask was opened on the ninth. Vibrios were found in 
abundance, of different lengths, some of them moving with 
great rapidity. 17 was boiled thirty minutes, the film was 
formed on the third, and the flask was opened on the ninth 
day. Vibrios were found in great numbers, some of them 
bending and extending themselves rapidly. Some minute 
spherical bodies were also seen, having the kind of motion 
which results from vibrating cilia, though none of these were 
detected. Exp. 18 was boiled fifteen minutes, the fluid having 
