WYMAN, ON THE FORMATION OF INFUSORIA. 115 
been previously filtered; the film formed on the third, and 
the flask was opened on the eighth day ; the organisms found 
were the same as in 17. Exp. 12 was boiled one hour, the 
film formed on the second, and the flask was opened on the 
twenty-fourth day. The infusion had a slightly putrid odour, 
and contained Vibrios and Bacteriums. 
Exp. 20. (2) March 22nd. The flask and the contents of 
it were the same as in the experiments just described. The 
solution was boiled fifteen minutes, the film formed on the 
fifth, and the flask was opened on the thirty-first day. The 
fluid had become of a dark reddish-brown colour, the film 
had disappeared, and some of the shreds of the coagulated 
albumen had become nearly black. Bacteriums were found 
in large numbers, and the darker shreds seemed to be made 
up of them. 
Exp. 21. (2) March 27th. Beef juice was boiled forty 
minutes, the flask was opened on the twenty-fifth day, and 
found to contain Bacteriums. 
Exp. 22. (2) April 2nd. Beef-juice and fragments of beef 
were boiled fifteen minutes, and the air was introduced through 
a much smaller tube. Bacteriums were found on the twentieth 
day. 
ep. 23. (2) April 5th. Was prepared in the same way as 
the preceding experiment. The film formed on the sixth day, 
and the flask was opened on the seventeenth. The sealed 
end was melted in the flame of a spirit lamp, when the gas 
escaped with force. Bacteriums were found. 
Expts. 24, 25, 26. (8) April 16th. Were all prepared in 
the same way. The capacity of the flasks was 550 c.c.; the 
contents were beef juice and water 17 c.c., urine 7 ¢c.c. The 
flasks were folded in a napkin, immersed in water, which was 
gradually heated to the boiling point, and each then exposed 
to it for thirty minutes. The film formed in 26 on the fourth 
day, and in 24 and 25 on the fifth, and were all subsequently 
found to contain Bacteriums. 
Expts. 27, 28. (3) April 24th. Two flasks, each of 550 
c.c. capacity, and each containing about 20 c.c. of beef juice 
and urine, were hermetically sealed at the temperature of the 
room, wrapped in cloth, and exposed for two hours to boiling 
water. The film formed on the fourth day, one of them was 
opened on the fifth, and the other on the eleventh, and both 
found to contain Bacteriums. 
Expts. 29, 80. (1) February 17th. In both of these the 
contents of the flasks were solutions of sugar and gelatine in 
water, to which fragments of cabbage leaves were added. 
The air was introduced through a Bohemian glass tube, filled 
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