116 WYMAN, ON THE FORMATION OF INFUSORIA. 
with asbestos and platinum sponge, and heated to redness. 
The materials were boiled thirty minutes. In 29 the film 
was formed on the twenty-ninth, and the flask opened on 
the thirty-ninth day. The solution was found to contain 
Bacteriums and cells filled with them. In 30 the film was 
formed on the seventh day, and Bacteriums were found on 
the twenty-third, when there was a slight odour of putre- 
faction. 
Expts. 31, 32, 33. (2) March 24th. Thirty grains of sugar, 
20 c.c. of beef juice, 158 c.c. of water, were divided into 
three parts, and each part put into a flask of 550 c.c. capacity, 
and boiled fifteen minutes. No film was formed in either of 
them. 383 was opened on the thirtieth day; ferment cells 
and some filaments of a doubtful vegetable appearance were 
found. 32 was opened on the forty-second day, and found 
to contain ferment cells in large numbers, in different stages 
of cell multiplication; as in 32 there was an escape of gas. 
Exp. 34. (3) March 27th. Juice of mutton, in a hermeti- 
cally sealed flask, was boiled five minutes in a Papin’s digester, 
under a pressure of two atmospheres. A film formed on the 
fourth day. It was opened several days later, in the presence 
of Prof. Gray, and found to contain Vibrios and Bacteriums, 
some of them moving with great rapidity. 
Exp. 35. (3) The same as the preceding, and boiled in 
Papin’s digester ten minutes, and under the pressure of five 
atmospheres. No film was formed. The flask was opened 
on the forty-first day. Monads and Vibrios were found, 
some of the latter moving across the field. No putrefaction ; 
the solution had an alkaline taste. 
Exp. 36. (3) March 28th. Beef juice was filtered and 
boiled, as in the preceding experiment, fifteen minutes, under 
two atmospheres. Opened on the forty-first day, and no 
evidence of life was found. When the end of the flask was 
heated, previously to opening, it collapsed. 
Exp. 37. (3) March 28th. The same as the preceding ; 
boiled fifteen minutes, under five atmospheres. Opened on 
the forty-first day, and no evidence of life was detected. 
We have here a series of thirty-three experiments, pre- 
pared in different ways, in which solutions of organic matter, 
some of them previously filtered, having been boiled at the 
ordinary pressure of the atmosphere for a length of time, 
varying from fifteen minutes to two hours, and exposed to 
air purified by heat. In four instances, viz., in expts. 4, 5, 
8, 9, the contents of the flasks were unchanged at the time 
they were opened; but in all of the rest, Bacteriums, Vibrios, 
or other organisms appeared. In nearly every instance their 
