458 J. R. BRADFORD AND H. G. PLIMMMK. 



size. We have never observed conjugation taking place 

 between the large hyaline forms described above. 



V. "Amceboid" and" Plasmodial " Forms of thk 

 Trypanosoma Brucii. 



We do not wish or mean to attach any pai-ticiilar biological 

 meaning to these two terms, which we use here only for 

 convenience of description. Besides the forms described 

 above, there remain two other forms which practically seem 

 to be of the greatest importance of any, as the death of 

 many of the animals we have used seems to depend upon 

 their presence. 



The first of these forms is that which for convenience 

 we have called '' amoeboid." By this we mean a form 

 consisting of a small irregularly shaped mass of protoplasm, 

 with a micro- aud macro-nucleus, and with or without a 

 flagellum ; they are all very mobile when examined in the 

 living state. This form is depicted in figs. 24 — 37. The 

 protoplasm is very delicate and uniform, and the micro- 

 :ind macro-nnclei are small, but very distinct; aud this 

 form is only seen well iu stained preparations when the 

 dilferential stain we have described above is used. But 

 they can be seen in fresh unstained preparations with very 

 careful critical illumination ; iu fact, our tirt:;t observation 

 of them was iu a fresh unstained preparation. 'J'lie flagella 

 may or may not be seen, but we believe that they are, 

 when free, all flagellated, but that iu the preparation of 

 the film the flagella may become detached, as free fl:igella 

 attached to their microuuclei are seen in most preparations 

 iu which these amcBboid forms can be found. They are 

 found in the blood from the lungs of most of the animals 

 we have used, especially iu that of the rat, mouse, cat, 

 and dog, where they are present in enormous numbers; 

 they are also found iu the glands iu small numbers, and 

 in the bone marrow of animals from which the spleen has 

 been removed before inoculation. They may also be 



