462 J. R, BRADFORD AND H. G. PLIMMER. 



with breathing. In the horse and mule the organisms are 

 constantly found in the blood, and the spleen is enlarged 

 and contains amoeboid forms. The sheath becomes swollen, 

 and the abdomen oedematous, and they die in about six or 

 eight weeks with paralysis. The Trypanosoma is longer and 

 thinner in these animals than in any we have used. The 

 guinea-pig shows the organism in the blood in varying 

 numbers and at varying periods; sometimes there are none 

 to be found for long periods, and again they may be nume- 

 rous for a time. The spleen is not enlarged, and the bone 

 Tuarrow contains no organisms. Amoeboid forms can be 

 found in the blood from the lungs. The guinea-pig may live 

 as long as eighteen weeks after inoculation. The pig shows 

 the organism in the blood very rarely and in very sm;dl 

 numbers, and dies with pulmonary symptoms. The spleen is 

 not enlarged. The spring-bok dies in about four weeks 

 with nervous symptoms, and with the organisms present in 

 the blood. 



It will be seen from the above that there is a good deal of 

 variation in the distribution of the Trypanosoma, and in the 

 form in which it is mostly present in the different animals 

 The rat and mouse show the greatest number and die quick- 

 est ; the guinea-pig and rabbit show fewer organisms, with a 

 small number of the amoeboid forms, and take much longer 

 to die ; the goat shows the least sign and is, in our experi- 

 ence, the most i-esistent animal. In those animals which live 

 longest the amoeboid and plasmodial forms are few in number 

 or absent. In certain animals there is a tendency to blocking 

 up of the lymphatics of certain regions (e. g. eye, sheath, 

 abdomen, and legs), and of lymphatic effusion around ; and 

 in others, a tendency to blockage of the brain capillaries 

 with amoeboid forms, which, in most animals, independent of 

 variations generally, is the proximal cause of death. 



In animals (rabbit, cat, dog, rat) from which the spleen 

 has been removed the distribution of the organisms is 

 different. During life there can be seen in the blood tangles, 

 consisting of numbers of the adult organisms writhing about 



