434 W. TIANNA. 



(fig. 1) it will be seeu that the organism is of large size, 

 showing a total length of 45 to GO f.i, and a breadth of 6 to 8 /u. 

 Stained b}' the Romano wsky method, it is seen to possess a 

 lai'ge centrally placed macronucleus extending the whole 

 depth of the parasite, and ranch deeper than long ; the micro- 

 nucleus or centrosorae is very small, and stains very deeply ; 

 close to the centrosome is a clear spot or vacuole. 



The protoplasm is not uniform in staining, but shows here 

 and there lighter coloured patches, and in some forms dis- 

 tinct evidence of longitudinal striation of the protoplasm 

 is observable. Scattered through its substance, especially 

 towards each end, are granules of a dark violet colour. The 

 anterior end of the body is much elongated, pointetl, and 

 gradually merges into the flagellum, so that it is difficult to 

 say where the body ends and the free portion of the flagellum 

 begins. The flagellum originates from the micronucleus, 

 and proceeding forwards along the outer side of the undulat- 

 ing membrane to the anterior end, it becomes free. This 

 free portion of the flagellum is exceedingly fine and attenuated. 



At the posterior end the body also tapers, and ends in a 

 pointed extremity, which stains a little less deeply than the 

 other parts of the protoplasm ; it has dark violet granules. 



Tlie undulating membrane is very distinct but narrow ; it 

 can be seen proceeding from the neighbourhood of the micro- 

 nucleus forwards to the anterior end of the body. 



Major Boss has kindly allowed mo to examine some slides 

 of the blood of crows made by him in 1898 in India. These 

 contain trypanosomata, but of an apparently different species 

 from that already described; their length, however, appears 

 to be about the same. 



These specimens from the crow (fig. 2) were faintly stained 

 with methylene blue, and owing to the length of time since 

 mounting they had somewhat lost their colour. I am there- 

 fore unable to describe them as minutely as I should have 

 liked, chiefly as regards the position and size of the macro- 

 nucleus. 



The chief points in the trypanosoma from the Indian crow 



