294 
Ittrinors NarurAL History SurvEY BULLETIN 
Vol. 26, Art. 3 
Table 7.—Longevity of conidia of Endoconidiophora fagacearum grown on sterile wood 
labels and stored in 100 per cent humidity at various centigrade temperatures. 
Per Centr GERMINATION AT DESIGNATED TEMPERATURE 
NuMBER 2 
or Days 
0° 12 
ae COS ks sateen 83 83 
LY RRP ene gee 80 70 
eto Gee 71 67 
Pe ok Seite 70 66 
7 pe ai 70 60 
|| Scat QO oh 55 33 
BR ES a5. ae 54 31 
7 es 49 17 
BM ie aris Re es 49 8 
7 Sell pare See. 45 7 
Lat rie are) cae 21 4 
(LD eae na a pyle past 10 2 
(TS eo OR aa 0 0 
Baie Ne) Jade, 0 0 
16° yg KY ig 34° 
83 83 83 83 
75 39 40 30 
67 31 26 6 
57 26 3 1 
32 18 0 0 
7 0 0 0 
4 0 0 0 
1 0 
1 
0 
0 
0 
Table 8—Longevity of conidia of Endoconidiophora fagacearum grown on sterile wood 
labels and stored in a dry atmosphere at various centigrade temperatures. 
Per Cent GERMINATION AT DESIGNATED TEMPERATURE 
NuMBER 
or Days 
0° 122 
an Sue oa dake 83 83 
jt Be ates Pinks ca 58 81 
CD HR tk ESS Rey 48 76 
x, | ae 9 a > 45 22 
= Raed ae pa 41 20 
gee ar ee 41 21 
= Miele: SURO oa 40 14 
Mee sitet oe: 38 14 
hy Re a ae a 33 13 
RO2 i Bee 30 12 
A ae Renae a para 31 10 
DOG Se ee a 22 il 
was recorded in a count of 300 spores. 
The remaining labels were transferred 
from the broth to sterile, dry beakers 
placed within 12 clean desiccators, 6 of 
which contained 200 ml. of sterile dis- 
tilled water and the others 200 gr. of cal- 
cium chloride crystals. These desiccators 
were arranged in pairs (each pair having 
one desiccator with water and one with 
calcium chloride), and each pair was sub- 
jected to one of the following tempera- 
tures: 0, 12, 16, 25, 31, and 34 degrees. 
Thus, the fungus was subjected to the ex- 
treme conditions of 100 per cent humidity 
and complete, or nearly complete, desic- 
cation at temperatures ranging from 
freezing to high. At intervals, one label 
16° 25" a1* 34° 
83 83 83 83 
74 54 7 2 
74 10 2 1 
29 s 1 0 
25 3 0 0 
paps 2 0 0 
20 1 0 
17 0 
14 0 
12 0 
8 
3 
was taken from each desiccator, and a 
spore suspension was prepared from the 
fungus on the label. Each suspension was 
adjusted to 300,000 spores per ml., and 
drops of it were placed on the surface of 
water agar within circles marked on the 
outside of Petri dishes. These dishes were 
incubated at 28 degrees for 36 hours; then 
300 spores were counted for each temper- 
ature and moisture condition tested and 
the per cent of germinated spores was de- 
termined. 
Viable spores were taken from labels 
stored at 100 per cent humidity and 0 and 
12 degrees temperature for 102 days, but — 
no viable spores were found on labels 
stored at these temperatures for 162 days, 
