September, 1955 
rot isolates +7-1, 50-7, and 50-22, and basal 
dry rot isolate +7-8 keyed out to F. euoxy- 
sporum. Brown rot isolates 45-74, 45-75, 
46-4, and 49-19 fell into F. orthoceras 
var. Jongius, and brown rot isolate 49-1 
and basal dry rot isolate +7-2 keyed out to 
F. moniliforme. Basal dry rot isolates 
46-12 and 49-20 keyed out to F. fili- 
ferum, but according to Coons & Strong 
(1931) this species decolorizes crystal vio- 
let. None of the isolates of the gladiolus 
Fusarium decolorized crystal violet. 
Although, on the basis of their reactions 
to the three aniline dyes, the majority of 
ForsBerG: Fusarium DIsEasrt oF GLADIOLUS 
461 
the isolates of gladiolus Fusarium fell into 
the F. oxysporum—F. orthoceras group, re- 
actions of so many of the isolates varied 
from typical reactions of this group that it 
would be impossible to use the aniline dye 
method of classifying Fusaria from gladi- 
olus with any degree of certainty. Neither 
would it be possible to separate isolates of 
the three disease forms, vascular, brown 
rot, and basal dry rot, on the basis of their 
reactions to aniline dyes. 
The results of this phase of the investi- 
gation agree with the results of Moore & 
Chupp (1952), who found that certain 
Table 5.—Reactions* of isolates of the gladiolus Fusarium to four concentrations of bril- 
liant green in Coons’s agar.} 
CoNCENTRATION OF Dye 
ISOLATE 
1:25,000 1:50,000 1:100,000 1:200,000 
See oe BRE RO Oe ee ere 0 tr 12.001 12.301 
ofidQe o doe bls US SS Se ree 0 0) 12.024 13.004 
b= Qe oe 26 aC eee ee ae 0) tr 12.001 13.001 
L525 5 5 oooh ee ea Sa en 0 0 11.001 12.001 
Cn ME en ot. ahd stom ane) 9 sicanadteee 0 tr 12.001 13.001 
Abc Ge pu) 2 oe See ge rn 0 0 12.001 13.004 
VAD sid BS Fa OS CI an Ea ee a a 0) 0 12.001 13.001 
LTD oe a Aaa ce Sen eae eee 0 0) 12.001 12.001 
edict oo A Gal Belen ee 0) 12.001 14.001 15.001 
DNS oars Sec § doe ENS ARG eee eee eee 0) 0) 12.001 13.001 
NB oe sileie oe ee ee 0 12.001 14.001 14.001 
1 HadlQs abc ote deh oe re 0 0) 12.014 13.001 
bis ilee oa oop a\Gs SERENE ee oe 0 0 11.001 13.001 
Gos Ste athe cls te Eee nee 0 0 12.001 13.001 
TEDAB 0 4 & ore leh Geen ee a ween 001 12.001 13.001 14.001 
SDs sas Pas ee = 0) 11.001 12.001 13.001 
DR s Be 2 ola ec ne 0 tr 12.001 13.001 
ci dictile oo leraudne Gea) Chauhan eee ae 0 11.001 12.001 13.001 
pa Ns 35s ON ee ene ee ea aor 0) 0 12.004 13.004 
2273 5 ee SSS ag ne 0) tr 12.001 13.001 
Se TI ye cacy: Aen Sei RIN a Raa 0 0 tr 11.011 
cjed): soos S Oo scales ae eee st ee 0) 11.014 12.014 13.001 
jollDo 5 4 She cc Reta Aree eee Oe 0) tr 11.001 12.001 
Talks oo pscvasunts eee ee 0 11.014 13.014 14.014 
Mee go b-y + ote Ste Rees LE oe an 0 11.014 13.001 14.001 
fPoMID., oA eMac cs eee Se 0 tr 12.001 13.301 
Ie aS oo ge SR ee 0) 11.004 13.024 14.024 
17 SY Shs ick ER ee I ae a 0) tr 11.001 12.001 
Lelia s sls 22 pata Sa eee eae 0 11.001 12.005 13.005 
Pea RPE eee seen av hime ances wideelip wing 0) 12.014 13.014 14.004 
FET ad A) I se RRR Se SE ee ie da dhe 0 12.004 12.014 13.004 
SUS ey osbe bs CRS ee ee 0 12.011 13.011 14.024 
EAD pe, Ee ES fe ta Pn a 0 11.011 12.005 14.005 
SUSIE) ols oe en ee, ea 0) tr 11.001 13.001 
ine NPI eer TRE AEM ces ce Or eg n.d 0 11.004 13.004 14.014 
1 able co a ble Te ee EE i are ee 0 11.001 12.301 13.301 
| 2h ep oe See eI Aen 0) 11.014 12.001 13.004 
+ 00 sos ots SUS Sts tet een ee 0 11.001 12.301 13.001 
“SRL +) Ways ON ae a a2 eae ae 0 tr 11.001 12.001 
ian (eT hier Cid FE aah tvce ce ws. ite se 0) tr 11.001 12.001 
*Reactions are expressed in the numbering scheme of Coons & Strong (1931), as explained in the text. 
{See table 3 for control readings. 
