464 
isolates of Fusarium causing wilts of to- 
mato, cabbage, and muskmelon did not re- 
act. toward malachite green and crystal 
violet in the way described by Coons & 
Strong (1931) for those species. 
Reactions to Copper Salts 
Coons & Strong (1931) used copper 
sulfate in the culture medium in some pre- 
liminary tests and expressed the opinion 
that this salt might be useful in identifying 
some species of Fusarium. Nelson, Coons, 
& Cochran (1937) reported that two 
Table 6.—Reactions* 
concentrations of copper sulfate in Coons’s agar.} 
CoNCENTRATION OF CopPpER SULFATE 
IsOLATE 
M/100 M /500 M/1000 | M/3000 
SSIS oe eee Bes 0 0) tr 12.301 
AS WO iis de eee 0 0 tr tr 
AG St et eee tr 11.329 12.301 13.001 
AG-5 Ae eee. eee 0 0 tr 12.304 
46-9. Serer ee 0 0 0 tf 
46- Ee ae ee 0 0 0 tr 
ATG Ree hee 0 0 0 tr 
ry LEU) pa ee 0 0) 0 12.301 
AO2ALT MRE a. tr 12.301 15.001 15.001 
ROADS ee es 0 0 0 0 
AO 295 i aay 45 - 0 tr 13.301 15.001 
A930) eo ed 0 0 0 0 
CBS ye ere, Cera tr 12.304 14.001 15.001 
BGs ee ee 0 0 0) tr 
LAE Uk Stat Mis fe 0 0 0 14.001 
LN 1g iy a aren tas tr 12.301 14.001 15.001 
BOIS count 0 0 0 tr 
SEER ed eas ee 0 0) 0 0 
coeds [Re aie aeet 11.309 13.301 15.001 15.001 
ASS te oe as tr 12.301 14.301 14.000 
ABET ee Ie Winton tr 12.301 13.301 13.301 
AG AGH a ers tr 12.301 14.001 15.001 
palo nthe ie 0 0 0) tr 
CTE etn See ea tr 12.301 13.001 15.001 
5 fa RE a 0 tr 12.001 14.001 
i 0S be Fata aes We tr 13.301 13.301 15.001 
7 Bh an I SRR tr 12.301 13.301 15.001 
HG fod dae en, ania es 0 0 0 tr 
BNE ae nets tr 132301 13.301 15.001 
ROMA Ries hier tr 12.301 14.001 15.001 
AG=D ase 5 Ae 0 0 0 tr 
BOE PA Aka pee 0 0 0 tr 
Sy age eee tr 13.004 15.004 15.004 
RODS aa ete 0 0 0 13.311 
SE BY A ae ep iae 0 12.301 14.001 15.001 
(Saale veut tr 12.301 14.001 15.001 
SSH eS 0 0 0 tr 
vO ES 1 oa Ne ea pe 0 0 0 11.001 
BOF ee ty 0 0 0 tr 
SOLE ii eo eee 0 0 0) tr 
* Except for .009 (clumpy) of author, reactions are expressed in the numbering scheme of Coons & Strong (1931). 
+See table 3 for control readings. 
forms of Fusarium which cause two forms 
Ittinois NaTturAL History SurRVEY BULLETIN 
Vol. 26, Art. 6 
of the yellows disease of celery could be 
differentiated 
tion, 
grams of copper sulfate (1 mole= 
if isolates of them 
grown on synthetic agar containing either 
copper sulfate or copper chloride. a 
The 40 isolates of gladiolus Fusarium 
used in the investigation reported her 
were grown on Coons’s agar to whic 
amounts of copper sulfate were added t 
make a series of concentrations that ranges 
from 1/100 molar (7/100) to 1 
molar, table 6. The strongest concer 
M/100, was prepared from i. 
M/5000 
11.300 
tr 
tr 
tr 
15.001 
15 001 
15.001 
15.005 
15.001 
tr 
tr 
of isolates of the gladiolus Fusarium to seven different molar ( 
M/6000 
15.005 
15.005 
tr 
tr 
249. 
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