Ittinors NATURAL History SuRVEY BULLETIN Vol. 26, Art. 6 
474 
Table 12—Readings in pH of Tochinai liquid inoculated with different isolates of the — 
gladiolus Fusarium; original pH 6.21. 
— 
Days Arrer INOCULATION 
ISOLATE tram 
Pee 5 8 12 15 2S 28 34 41 50 58 
BaD uetins’s: «aes ke oe 5.68 | 5.25 | 4.87 | 4.84 | 4.97 | 6.99 | 7.45 | 8.36 | 8.48 | 8.47 | 8.32 
AS“ BO ssid cc polaris arte 6.10 | 4.78 | 4.94 | 5.52 | 6.06 | 7.10 | 7.68 | 8.27 | 8.52 | 8.52 | 8.47 
UPS ALES neta 8 Pa 5.63 | 5.10 | 4.93 | 5.30 | 6.03 | 6.50 | 7.38 | 8.29 | 8.54 | 8.58 | 8.45 
7 (Es ens ae aa SL EOE Here 5.51 | 4.85 | 4.73 | 5.60 | 6.65 | 8.49 | 8.72 | 8.72 | 8.55 | 8.15 | 8.59 
Gta Davai, stertts 5.50 | 4.84 | 4.75 | 5.49 | 6.06 | 6.85 | 8.22 | 8.63 | 8.61 | 8.48 | 8.41 
A614 fo weg e 5.55 | 4.83 | 4.78 | 5.78 | 6.03 | 6.57 | 7.90 | 8.40 | 8.59 | 8.54] 8.45 
LIE epee eatery ere 5.60 | 4.78 | 4.63 | 5.52 | 5.84 | 8.53 | 8.50 | 8.61 | 8.48 | 8.32 | 8.12 
BLO Rn toe ey 5.33 | 4.78 | 4.63 | 5.73 | 6.68 | 6.77 | 6.47 | 8.21 | 8.44 | 8.54 | 8.61 
V2 ERE eect ae 5.55 | 4.90 | 4.95 | 6.20 | 6.47 | 6.32 | 7.53 | 8.44 | 8.62 | 8.42 | 8.45 
QUO al thay va cape atope ene 5.78 | 4.92 | 4.90 | 6.48 | 7.03 | 6.25 | 7.07 | 8.31 | 8.46 | 8.50 | 8.38 
LOS d Pe eat eee 5.94 | 5.30 | 4.88 | 5.79 | 6.88 | 7.93 | 8.40 | 8.58 | 8.52 | 8.32 | 8.15 
2 oF peat ea EBe Et 5.27 | 5.03 | 4.83 | 4.98 | 6.00 | 7.50 | 8.02 | 8.53 | 8.51 | 8.44 | 8.43 
SO Dee cite bree es 5.59 | 4.85 | 4.89 | 6.08 | 6.42 | 6.68 | 6.98 | 8.45 | 8.48 | 8.65 | 8.62 
SAM esata feces 5.41 | 4.92 | 4.95 | 6.38 | 6.98 | 8.37 | 8.50 | 8.50 | 8.40 | 8.23 | 8.09 
BOLD R hase Bs acne cas 5.55 | 4.75 | 4.61 | 5.38 | 6.07 | 6.53] 6.71 | 7.82 | 8:45 (8755 eee 
SE Bie ee Nore 5.60 | 5.00 | 4.88 | 5.89 | 7.38 | 8.49 | 8.55 | 8.45 | 8.47 | 8.32 | 8.15 
eA ete cig ta 5.70 | 5.03 | 4.92 | 5.95 | 6.67 | 6.63 | 7.58 | 8.37 | 8.43 | 8.61 | 8.50 
Nea) hs Ps ceiee ea pases 5.45 | 4.88 | 4.91 | 6.18 | 7.00 | 6.98 | 7.32 | 8.19 | 8.44 | 8.51 | 8.40 
2 oS |S ine eae 5.88 | 5.21 | 4.78 | 5.61 | 6.70 | 8.34 | 8.37 | 8.48 | 8.58 | 8.55 | 8.38 
BG PA Fe er Atos 3 5.75 | 5.45 | 5.27 | 5.58 | 6.26 | 7.33 | 7.33 | 8.40 | 8.48 | 8.64 | 8.51 
eV ps NPM se ie ee ea 5.51 | 4.74 | 4.60 | 5.12 | 5.50 | 5.93 | 6.17 | 8.20 | 8.42 | 8.58 | 8.45 
ol Naar cease Mert Rei 5.69 | 5.05 | 4.93 | 5.55 | 6.38 | 6.95 | 7.84 | 8.48 | 8.59 | 8:50 ieerae 
A] = a oi Cea ae 5.59 | 5.27 | 5.04 | 5.91 | 6.38 | 6.77 | 7.61 | 8.37 | 8.574 8.65 eee 
7 Fo ESL xe tc ete at 5.64 | 5.18 | 4.94 | 4.90 | 5.10 | 6.55 | 7.59 | 7.48 | 8.43 | 8.58 | 8.45 
ATEN acres eats 5.68 | 5.12 ; 5.00 | 4.96 | 5.24 | 6.60 | 7.54 | 8.08 | 8.62 | 8.49 | 8.42 
AD Dita te o b eres: 5.69 | 5.12 | 4.86 | 5.16 | 6.15 | 8.28 | 8.40 | 8.62 | 8.48 | 8.44 | 8.28 
Zo BA, Ce Be teeta et 5.83 | 5.10 | 4.89 | 5.75 | 6.70 | 7.19 | 7.35 | 8.48 | 8.61 | 8.59 | 8.41 
AEN ae e Siatig es 5.42 | 4.89 | 4.77 | 6.00 | 6.67 | 7.29 | 7.44 | 8.29 | 8.54 | 8.54 | 8.46 
AOD Mesa) Soin aye Rie oe 5.53 | 4.95 | 4.89 | 6.40 | 7.10 | 6.47 | 8.15 | 8.40 | 8.52 | 8.62 | 8.47 
SOS ee Van neste 5.50 | 5.21 | 5.07 | 5.96 | 6.17 | 6.70 | 7.27 | 8.40 | 8.64 | 8.49 | 8.46 
bs 9S Aap ate gia ee eaten 5.66 | 4.95 | 4.78 | 5.74 | 6.73 | 7.48 | 8.06 | 8.44 | 8.43 | 8.50 | 8.52 
5 USO eg meee cs eee ene 5.48 | 5.09 | 4.95 | 6.50 | 6.85 | 7.51 | 7.69 | 8.29 | 8.60 | 8.65 | 8.49 
LG Pah Se kesh ee a 5.72 | 4.87 | 4.55 | 5.02 | 5.81 | 7.15 | 8.02 | 8.48 | 8.37 | 8.60 | 8.48 
2 he FANS RAR oe RRR re 5.51 | 4.90 | 4.88 | 6.15 | 7.08 | 7.94 | 8.39 | 8.45 | 8.55 | 8.58 | 8.42 
gO EC Serna bere ieateneey 5.72 | 4.90 | 4.62 | 5.67 | 6.38 | 6.32 | 6.10 | 8.23 | 8.41 | 8.47 | 8.51 
BODO Wipaiedaes aca ee 5.53 | 4.93 | 4.80 | 6.13 | 6.64 | 6.52 | 7.38 | 8.32 | 8.43 | 8.65 | 8.59 
DOLLS or ures ea 5.52 | 4.85 | 4.71 | 6.04 | 6.60 | 7.34 | 6.50 | 8.36 | 8.39 | 8.56 | 8.43 
BUDO ree he hee 5.67 | 5.20 | 4.85 | 4.83 | 5.68 | 8.00 | 8.48 | 8.48 | 8.38 | 8.33 | 8.23 
pH changes and virulence is exactly oppo- 
site to that reported by Wellman for the 
tomato Fusarium. 
In fig. 164, brown rot isolates 47-12 
and 50-25 are compared. Isolate 47-12 
was of the raised type on Wellman’s agar ; 
50-25 was intermediate-raised. Isolate 
47-12 produced rot in inoculated corms; 
50-25 did not. The pH curves of the two 
isolates show that in cultures of both iso- 
lates the same low point was reached at 
about the same time but that the rise in 
pH was much sharper in cultures of iso- 
late 50-25 than it was in cultures of iso- 
late 47-12. With isolate 50-25, neutrality 
was reached in about 17 days but, with 
isolate 47-12, not until after 25 days. 
Brown rot isolates 9-17 and 49-19 are — 
compared in fig. 16B. Isolate 49-17 was — 
of the appressed type, and isolate +9-19 
was intermediate-raised. Isolate 49-17 
produced corm rot in laboratory tests in 
1952 and 1953; isolate 49-19 produced — 
corm rot in 1952 but not in 1953. The © 
pH curves for these two isolates are sim- 
ilar except for a second drop in pH from ~ 
the fifteenth to the twenty-third days in 
the culture of isolate +9-19. 1 
Vascular isolates 45-73 and 46-3 are — 
compared in fig. 174. Isolate 45-73 was 
the more virulent in greenhouse tests in — 
1948 and 1953. In 1952, isolate +6-3 was © 
slightly more virulent. Isolate 45-73 was — 
of the raised type; 46-3 was appressed. — 
