70 College of Forestry 
globose swellings. _Ungerminated spores usually became 
2-vacuolate. Germination in 0.5 per cent ethyl alcohol was 
greatly retarded — even more so than by the use of ether — 
and confined entirely to a few spores. Subsequent growth 
and behavior of the mycelium was the same as described for 
the use of ether. Germination in a 0.5 per cent solution of 
dipotassium phosphate was retarded to about the same extent 
as by alcohol, but occasional germination was to be seen. 
The growth of the mycelium was poor and highly irregular. 
After the escape of the protoplasm from the spore, through 
the germ tubes, it was noticed in a few instances that a cross 
wall was formed through the center of each spore. Spores 
germinated and grew vigorously in a 0.5 per cent solution 
of sodium chloride (Plate X). No germination could be 
observed in the following solutions: 0.5 per cent potassium 
nitrate, 1.0 per cent tartaric acid, 1.0 per cent ammonium 
phosphate, 1.0 per cent sodium carbonate, and 1.0 per cent 
citric acid. 
Vitality of Dessicated Spores.—A_ plentiful supply of 
spores was shed on waxed paper and the paper was folded 
up and filed away in a desk on November 8, 1915. It was 
found that these spores germinated readily after being kept 
in this condition for two weeks and it seemed desirable to 
obtain data on the duration of vitality of dessicated spores. 
As a result of germination tests made at various intervals 
from these spores, using hanging drops containing a 5 per 
cent solution of Merek’s malt extract, they were found to 
germinate as readily at the end of six months as when first 
shed. The growth of the mycelium, however, was very rapid 
and profuse branching occurred. The spores were again 
tested at intervals and were found to germinate readily at 
the end of ten months. Further tests were not made until 
twelve months had elapsed since the spores were shed. ~ At 
this time several tests for germination all resulted in failure. 
The dessicated spores swelled up in the nutrient solution as 
they had done before, but not a single case of germination 
was observed. 
