668 H. St. J. K. Donisthorpe and W. C. Crawley on the 
On July 13th Wes, of eggs had been laid in the 
Juliginosus nest, and on the 23rd a 2 with her abdo'~en 
enormously dilated was noticed in the damp chambc. of 
the nest, with a large pile of eggs surrounded by 9@. 
It may be as well to record that a winged /uliginosus 9, 
introduced into the fuliginosus nest, from Oxshott on June 
Ist, was at once killed by the 9%. 
Experiment No. 3. 
On December 10th, 1910, at 1.25 p.m.,a single winbratus 
% from the Weybridge colony was placed in a glass-topped 
box with a fertile fuliginosus 9. The 9 crept up behind 
the 2, touched her abdomen with its antennae, then drew 
back. The % again advanced with open mandibles 
several times, but did not actually attack the ?, who 
remained perfectly quiet. 1.32 p.m. another § was put 
in, which behaved in a similar manner to the first. 
One of the $6 examined the ?, but neither saluted her, 
though she seemed quite friendly. 1.39 p.m. a third & 
was put in. 1.41 pm. by way of contrast a winged 
umbratus & from the same colony was put with them. 
The 95% saluted her, and the /uliginosus 2 behaved to her 
just as she had behaved to the $9. Then a & got on the 
Juliginosus °’s back and bit savagely at her: she shook off 
the 6 and ran away. At 1.50 pm. the umbratus 2 was 
removed, and ‘the fwliginosus left alone with the three 
wumbratus 93. 1.52 p.m. another ¢ was observed to bite at 
the @, who again ran off. 
Next morning the four ants were found close together 
in the box, apparently quite friendly; so at 11.45 am, 
the three $9 were restored to their nest (No. 2 wmbratus 
nest), and the /wliginosus 2 put in after them. Ants came 
up to her with threatening jaws, but did not actually 
bite her. Later, however, one or two got hold of ber 
legs, but, except in two cases, did not hold on for long. 
Once an ant saluted her: this may have been one of the 
three that had been confined with her since the previous 
day. At 12.30 pm. a flavus 2 (fertile) was introduced 
into the nest for comparison. To our surprise she was 
not at once attacked, but soon a 6 fixed on her antennae, 
and held on so persistently that the 2 was removed. 
Between 1.9 p.m. and 8 p.m. the /uliginosus 2 was observed 
to be attacked four times. Whenever she was held by a 
leg it was interesting to see how patiently she stroked the 
