236 



expect that the extract with water should always show much lower 

 values than the extract with the culture solutions. This is by no 

 means always the case; the former is often even higher than the 

 latter. Again the diastase values in the culture solutions agrée very 

 well. There may be important variations on the same day, but the 

 gênerai course of events in both is the same. Thèse facts support my 

 view that the quantity of diastase m the mycélium is only accidentai. 



Fig. 2. Diastase in mycélium -^ culture solution ( — 



solution only ( ), (d = days)l 



); diastase in the culture 



The enzyme is secreted in the first stages of active development, 

 so that I can not favour the view that the diastase in the liquid is 

 only derived frcm cells, which hâve died and allowed the enzyme 

 to pass out. I feel sure that the diastase has passed to the outside 

 through the living protoplasm.a. 



