244 



the culture solution were mixed, cne with an equal volume of water 

 and the other with an equal volume of 0.08% staich solution. In 

 this way the H m both cases was equal. The mixture to which 

 starch had been added, took a longer time to give a négative iodine 

 test. This différence of time was taken to be a measure of the 

 amount of diastase présent in the original solution. It is an open 

 question, whether this method is quite accurate. As howcver it 

 is only usedduring thefirst few days of development, during which 

 there are no important events in the sécrétion of diastase, I thought 

 that there is very little to be said against using this method. 



Starch 2%. (11-22; 493) fig. 9, table 5. 



During the stérilisation of the culture solutions, a small preci- 

 pitate of starch came down. This was weighed in a few cases; the 

 average weight of the sédiments was found to be about 8% of the 

 total amount of starch used. This therefore can not influence the 

 expenment materially. 



2 4 6 8 10 



Fig. 9. Starch 2%. Diastase in the cuhure solution, (d =- days). 



The curve of the formation of diastase in this case is very similar 

 to the curves, given for the séries on 4 and 2.5% glucose. Develop- 

 ment however was slow, so that the maximum dry weight was 

 reached later. The curve reaches its minimum only on the 1 1 day. 



Starch 0.5% (7-11; 155) fig. 10 ( ), table 6 A. 



A A i. e. inoculated with conidia from a culture on starch 0.5%. 



