254 



For the 2.5% séries (11 — 13, 615.5) the same holds good for 

 the 5 — 18 day; 12 days after filtering, the following amounts 



were le: 



ft: 



in the solution of the 5*'' day 70 % 



One can already see the influence of the increasing dry weight. 

 This is also the case for the séries on 4% glucose. After 10 days 

 the following amounts of enzyme were left in the liquids obtained 

 from the cultures between the 5 and the 18 days: 



In this séries also the liquids from later days were not quite con- 

 stant. In most cases 20 to 30% of the diastase had been lost after 

 about two months. 



In the case of the 5% glucose séries (Il — 19,- 944.5), only the 

 solutions of the last few days hâve been kept. From the curve in 

 fig. 4 it can be seen, that ail the enzyme is never set free. It is 

 therefore quite in accordance with this fact, that even thèse solutions 

 should loose their strength very rapidly. After 6 days they retained 

 only the following amounts of their enzyme: 



the solution of the 33*»' day 8% 

 37*»' „ 9% 



40*»' „ 50% 

 46*»' „ 10% 



We may therefore safely assume, that none of the liquids of this 

 séries would hâve kept their strength for any length of time. 



