257 



this, we are not surprised that the results, described above, show 

 so many irregularlties. More over tables 7 — 9 show, that indi- 

 vidual différences between the diastase concentrations of two cul- 

 tures, exammed on the same day, are greatest on those days, where 

 we should expect the strongest sécrétion of inhibitory substances. 

 Just at présent, I am as yet unable to give the quantitative rela- 

 tions between the mass of inhibiting substance and the mass of 

 enzyme that is bound by it. I hâve attended to do by salting out, 

 by précipitation and by other methods, but without resuit. I can 

 only State positively that we hâve to deal with very small quanti- 

 ties hère. The liquids containing diastase were perfectly clear 

 without exception. (NH4)2S04 even in high concentration gave 

 no perceptible sédiment. 



IV. SERIES ON SACCHAROSE. GLYCERINE 

 AND LACTOSE. 



The séries under discussion now, were rather unsuccessful, as 

 far as diastase production is concerned. Yet they show some very 

 remarkable points. 



Four séries were grown on a solution containing 5°o saccha- 

 rose, viz. 



one inoculated with conidia from a culture on glucose 5%. 

 » » ., „ ,, „ „ ,, saccharose 5% 



and two similar parallel séries, but to which were added small 

 quantifies of ZnS04 and FeSOj (0.0025'^o,' Raulin). This was done 

 because I wanted to try, if the wellknown influence of thèse salts 

 on the dry weight, would also influence the production of diastase. 

 Ail thèse séries formed heavy mycelia, which showed large un- 

 dulations on their surfaces. The cultures to which iron and zinc 

 salts had been added showed a rather dark lemon colour, due to 

 the iron. The mycelia in thèse séries were very brittle and could 

 not be removed from the flasks in a single pièce as they broke 

 easily. Formation of conidia m thèse also was scanty and only 

 along the sides. The cultures which did not contain zinc and iron 



