260 



Development was very slow in the beginning. On the 7 day 

 the results as a whole were négative; on the 14 day the degree 

 of acidlty and development of mycélium were such, that a good 

 production of amylase could hâve been expected; yet none was 

 found. So we assume that not only the concentration, but also 

 the chemical constitution ot the cane sugar made itself felt. The 

 amounts of diastase that did appear, were very small and may be 

 attributed to the influence of the 4% glucose solution on which 

 the conidia, I started off with, were grown. 



On the 2P* day however, the results were entirely as I had ex- 

 pected them to be. The quantity of diastase found in the culture 

 solution was, it is true, not quite as great as on 1% glucose; yet 

 there was such a great rise in diastase production, that I do not 

 hesitate to accept this as an absolute confirmation of my supposi- 

 tion mentioned above. 



M i c h a e 1 i s and M e n t e n hâve determined what influence admix- 

 tures hâve when they are added to the combination enzyme- 

 substratum i. c. invertase-saccharose. They found that there are 

 substances, which greatly diminish the affinity of the enzyme to 

 its substratum. The split products of cane sugar did so to some 

 degree, glycérine for instance had a much stronger influence. 

 Perhaps we must consider saccharose and fructose as such admix- 

 tures in the starch-diastase combination. This fact would also ex- 

 plain why there was no diastase production on 5°o saccharose: this 

 experiment was not continued long enough to exclude ail inhibitory 

 f actors, first the saccharose and fructose, later on the great weight 

 of the fungus. If once we succeed to overcome thèse sources of 

 errors, the production of diastase can be observed as happened in 

 the small experiment on the 1% saccharose cultures. 



Glycérine 5% (28—33; 1021) table 13 A 



inoculated with conidia from a culture on 5% glucose; 



Glycérine 5% (29; 998) table 13 B 



inoculated with conidia from a culture on 5% glycérine. 



Development on glycérine was very slow; in the end however 



