REPORT OF SOCIETY 55 



urediniospores or in separate tcUa. It cannot be said that the tehospores are 

 produced only in the elongate sori,, as many were found in the round sori com- 

 monh' considered to be uredinia. The teliospores are thick-walled and two- 

 celled with a slight constriction of the spore wall at the septum. Thej^ are 

 darker than the uredinio-spores and usually bear persistent pedicels giving them 

 a club-shaped appearance. 



Scope of Experimental Work 



Peltier (6, page 538) writes : "All efforts to germinate the teliospores have 

 failed. The spores were subjected by the author to alternate wetting and drying 

 to high and low temperatures and to various outdoor conditions through the 

 winter and summer. The gelatine plate method was also tried under different 

 conditions, but with no success.'' Xo details are given concerning the lowest 

 temperatures to which the spores were subjected in the outdoor conditions. 



In order to corroborate these results, in part, hanging drop cultures of 

 teliospores which had been frozen for one daj^ were made in distilled water. 

 After one day 5% of the teliospores had germinated from one cell, usually the 

 apical cell. After two days 14^ had germinated with the production of four- 

 celled promycelia, some bearing sporidia on short sterigmata. This result led 

 the writer to carry on further tests to determine, if possible, the maximum ger- 

 mination after the spores had been subjected to outdoor conditions for varj'ing 

 periods. 



Other experiments to determine whether the rust was autoecious or heter- 

 CEcious were also conducted, but the writer was unable to complete them. 

 However, the results obtained to date are given here. 



Experiments in Germination 



All hanging drop cultures were made with ring cells and incubated in a 

 moist chamber at room temperature. The most uniform results were obtained 

 by the use of distilled water and tap water. Spore cultures were also made in 

 1% saccharose and 1% destrose solutions, but only slight germinations were 

 obtained. The results of the experiments may be tabulated as follows : — 



Per cent of Germination 



16% 

 12% 

 22% 

 14% 



