REPORT OF THE SOCIETY 81 



1. Natural — (a) without agar — string beans, raspberry cane, potato, white 



carrot plugs and steamed rice, 

 (b) with agar — iris leaf juice, potato juice, bean, and soil 

 solution agars. 



2. Synthetic . (a) without agar — Uschinsky's, Richard's modified, Dug- 



gar's and Harshberger's nutrient solutions, 

 (b) with agar — dextrose 2%, dextrose 10%, laevulose 2%, 

 Richard's and Uschinsky's solutions and Harshberger's 

 with 2% dextrose. 



Source and methods of isolation. 



The species of Fusarium studied in this work were obtained from the roots, 

 of wilted aster, garden and sweet pea, and lupine. In addition, for purposes 

 of comparison, three cultuies were obtained from outside sources. The source 

 of the different organisms in isdicated in tho table following: 



D — Sweet pea roots. 



F — Aster stem (just below surface of soil). 



G — Garden pea stem (obtained from Maine). 



H — Aster stem (obtained from Maine). 



J — Bean roots (obtained from Cornell). 



K — Lupine roots. 



L — Lupine roots. 



N — Lupine roots. 



G is a culture of F. orthoceras A & W., and H is F. conglutinans Woll., both 

 originally isolated by Lewis (7: 1913) and obtained through the courtesy of 

 Dr. W. J. Morse, of Maine University. J is culture of F. niartii phaseoli Burk., 

 originally isolated by Burkholder (2: 1919) and secured from Cornell Univer- 

 sity. F was isolated from a wilted aster in a garden in Notre-Dame de Grace, 

 Montreal. The remaining organisms were secured in the vicinity of Ste. Anne 

 de Belle vue. Que. 



In the isolation woi k every effort was made to secure absolutely pure cul- 

 tures. The roots of the wilted plants were washed and placed in moist chambers. 

 A superficial growth of mycelium appeared over the surfaces. Tiansfers were 

 made from different parts of the root systems (in case a number of species might 

 be present) to tubes of agar. When there appeared to be more than one organism 

 present the cultures thus obtained were carried for several weeks and transfers 

 made. 



In order to secure absolutely pure cultures, final isolations were made from 

 each culture by means of the poured plate method. The transfers were made 

 from single colonies when they appeared visible on the surface of the agar under 

 a pocket lens (20 x). In most cases the cultures thus obtained were found to 



