116 PROTECTION OF PLANTS — 1922-23 



Agar Plate. 



Small circular shining colonies were produced averaging in size about one 

 half mm. 



Gelatin Stab. 



There is an extremely abundant surface as well as stab growth, which is 

 beaded to filiform in appearance. Although the group numbers show lique- 

 faction in all cases, there is a vast difference in the cultures. The 19b derivitives 

 show fairly rapid liquefaction of the stratiform type starting the third day and 

 becoming complete at the end of two weeks. The 7al derivitives show extremely 

 slow liquefaction, never commencing before two weeks and £kt the end of five 

 weeks showing only a slight napiform appearance. With B. Cavotovorus how- 

 ever, the liquefaction is extremely rapid, being complete within six days after 

 inoculation. 



Gelatin Plate Colonies. 



Colonies are small and circular, and appear somewhat wrinkled when exa- 

 mined under the microscope. 



Potato Plugs. 



The growth is fairly abundant covering almost the entire the with a grayish 

 white growth. The mediara, however, was only very slightly grayed. 



Miscellaneous Features. 



All organisms showed abundant growth at 37°C but 27°C appears to be the 

 optimum temperature. In no case was there growth on Cohn's solution, but 

 Uschinshy' solution was favourable. 



Milk. 



The production of an acid curM with a slow peptonization of the casein was 

 always characteristic. 



Biochemical Features. 



In every case there was a production of ammonia and a reductionof nitra- 

 tes, also a slight production of indol. 



In conclusion, although the writer's isolated organisms have practically 

 the same group number as Bacillus cavotovorus, they are not identical, as can 

 be seen by the above characteristics, principally in relation to their liquefaction 

 of gelatin. Although the derivatives of 19b liquefy gelatin, the action is not so 

 rapid as in the case of B. cavotovorus, nevertheless the liquefaction cannot be 



