REPORT OF THE SOCIETY 127 



Setae were observed to arise from the sclerotia of all the organisms at some 

 time or another. In the case of the Quebec organism and Vermicularia varians 

 setae were found present in all the cultures while in the case of Colletotrichum 

 tabicjium, Colletotrichum atramentarium and Colletotrichum atrovirens setae were 

 not present at all times and when present were fewer and much shorter. The 

 last two organisms named have been cultured on artificial media for a much 

 longer time than the others and the writer attributed the non appearance of 

 setae to this fact. The setae arising from the sclerotia of the Quebec organism 

 and Vermicularia varians varied in length from 80 - 120u; the width near the 

 base was from 2-2. 6u, and tapered to a fine point. The oldest setae are usually 

 2-4 septate, black in color and arise from the centre of the sclerotium. When 

 young, they are light colored towards the tip. 



Spores of the six organisms were germinated in tap water in hanging drops. 

 Germination was similar in each case. Each spore produced a single germ tube. 

 Germination was typically acroblastic, but in cases where it was pleuroblastic 

 the germ tube originated nearer the apical end. 



On Liquid Media. 



The organisms under observation grew well on suitable liquid media, but 

 the chracteristics discussed above were, on the whole, very much slower in 

 development, and did not prove as striking in appearance as those displayed 

 on the solid substrata. 



On liquid media, the same similarity in type of growth was observed, the 

 Saltation being the only one that could be differentiated from the others on any of 

 the media used. It could be picked out by the fact that only white submerged 

 mycelium developed and no sclerotia appeared. There were three main stages 

 in the growth of all cultures ; first, the formation of white submerged mycelium, 

 second, the formation of a surface layer of mycelium and third, the appearance 

 of sclerotia on the surface layer. After the formation of the surface layer the 

 pink coloration (described later) was evident before the black sclerotia were 

 formed. No growth resulted in flasks containing Knop's solution, and from 

 this fact it would appear that sugar is essential to growth. 



Rate of growth. 



The following experimental work was carried on to determine and compare 

 the rate of growth on solid media of the five organisms. Vermicularia varians 

 has been omitted from this study as a culture of it was not available at the 

 time the work was done. 



The methods adopted were as follows: 



1. Petri dishes of uniform size (90 mm. in diameter and 14 mm. in depth) 

 were chosen and sterilized in a hot air oven in the usual manner. 



