REPORT OF THE SOCIETY 51 



No. Reaction to culture 1. 



61 . Full Measure Bushel. CS. 



62 . Mighty Nice. CS. 



63 . Plentiful French. CS. 



64 . Non Pa;reil. S. 



65 . Ne Plus Ultra. CS. 



Inoculation Experiments. 



After a few preliminary lots of beans had been inoculated to study the 

 conditions necessary for infection, culture No. 1 was used to inoculate the 

 varieties of beans received from Ste. Anne de la Pocatiere and from these results, 

 which are given with the list of vaieties, sixteen were selected largely on account 

 of their showing resistance or types of resistance. One completely susceptible 

 and three susceptil-le were included, the aim ]:)eing to get a variety of reactions 

 to the various cultures and at the same tin:e test out those resistant as far as the 

 tin.e available allowed. All the cultures sporulating at the time of making the 

 inoculations were used on the sixteen varieties. 



The beans were grown in pots in the greenhouse on benches and were 

 inoculated as soon as the first pair of true leaves had spread out. Such early 

 inoculation was to elin inate any danger of differences of resistance from the 

 ageing of the tissues. This plan n ade it necessary to inoculate on different 

 days; 48 hours being the longest apart of the inoculations on any one variety. 

 In addition to the age of the tissue as a factor in resistance it was later 

 found that ordinary fluctuations in weather conditions produced an error just 

 as great, though more easily determined. 



Inoculations were made by carefully spraying all parts of the seedlings with 

 a turbid suspension of spores with an atomizer. All spore suspensions were 

 made equally turbid and the atoujizers were sterlized, after each culture used, 

 in 60 per cent alcohol for at least five n.inutes. 



The plants were placed in a n:oist chamber after inoculating and kept in a 

 greenhouse norn ally run at 70 F. At the end of 48 hours they were removed 

 and placed on benches and observations made each dav, until the tenth after 

 inoculation when the final records on the degree of infection were taken. 



Signs of infection were usually easily seen on the sixth day though it ran- 

 ged from early on the fifth to the seventh. In son e cases of extreme suscepti- 

 bility, the plants had completely wilted and fallen o\'er on the sixth day. This 

 condition was peculiar as there were no signs of the typical brown lesions; just 

 water soaked areas being present on the stem on which, in a day or two, acervuli, 

 producing-spores, showed as I rown flecks. This early wilting and dying must 

 not be confused with the wilting that was so often evident when the plants were 

 first taken from the humid atmosphere of the moist chamber. This latter 

 condition was never present after the first 24 hours and the plants appeared per- 

 fectly healthy until the sixth or seventh day when they again wilted and died 

 completely if they were a susceptible variety. 



For convenience in taking records letters were used indicating the degree 



