REPORT OF THE SOCIETY 63 



ticta reported by Saccardo and considered that he did not have Phoma subcirci- 

 nata E. & E. reported in 1892 and worked on by Halstead, as he states that the 

 descriptions do not fit. In his inoculation experiments he used and infected 

 wax and kidney beans, lima beans and cowpeas, and states that infection is 

 entirely confined to the leaves. Smith's article seems to be the only one that 

 gives a description of the organism and is not as full as one might wish so a brief 

 study of the organism isolated was made. 



History of culture. 



Last autumn requests were sent out for bean pods affected with anthracnose 

 a,nd an:ongst other san^ples, one was received from a friend in Central Clarence, 

 Annapolis Country, N.S., who was not familiar with diseases of plants in general. 

 The saniple was accompanied by a statement to the effect that it had been 

 difficult, to secure spotted pods as there was very little disease on any of the 

 beans. On opening the sample a large number of pods were found with very 

 tiny flecks, but amongst them was a pod that had very large lesions which 

 appeared identical with those produced by Colletotrichum lindemuthiajium, 

 excepting i n the matter of size. Pieces of this severely affected pod were planted 

 in plates of potato dextrose agar but the only result was an organism that 

 developed rapidly and had a whitish superficial type of growth. This was set on 

 one side in the hopes that later it would be possible to identify it. Spores were 

 not in evidence until after Christn.as when what looked like pinkish rhizomorphs 

 were noticed penetrating into the substratum from the lower surface on the 

 mycelial mat. These on investigation proved to be masses of spores less than 

 half the size of those of C. lindemuthianum. A spore suspension was made in 

 sterile water and diluted until it was estimated that 2cc. would contain about 

 25 spores and this amount was poured on the surface of plates of potato dex- 

 trose agar. The resulting colonies were different from the original but all had 

 the same appearance and macroscopically were of the general Col'etotrichum 

 type. Transfers were made to agar slants and the organism studied in pure 

 culture, the cultures being carried under identical conditions with those of C. 

 lindemuthianum. 



The organism. 



Upon examination the main characters of this organism were found to 

 come fairly close to Saccardo's description of Phyllosticta phaseolina Sacc. 

 which is as follows; "Maculis amplis, vagis arescendo ochraceis; peritheciis 

 sparsis, lenticularibus, 70 u diam. pertusis; sporulis ovoideo-oblongis, 6 by 2.5, 

 rectis, rarius insequilateralibus, hyalinis". Smith gave the description of the or- 

 ganism that he works with as follows; "Spots irregularly scattered, subcircular, 

 2-10 mm. in dian.eter, deep rusty brown, becoming lighter in the centre, and 

 margin darker. Perithecia scattered, lenticular, 70-90 microns in diameter, 

 sporules ovoid oblong, mostly straight, 4-6 x 2-2.5 microns. On leaves of 



