REPORT OF THE SOCIETY 81 



and then placed in a hanging drop to which was added a small bit of a potato 

 leaf. After some da^^s some changes were observed in the protoplasmic contents. 

 In two cases spherical hyaline bodies suggestive of zoospores were seen moving 

 inside some of the bodies. They were carefully watched but nothing further 

 happened. Apparently it was but streaming of the protoplasm. 



Interesting results were also obtained by growing the fungus in liquid 

 extract of oatmeal. Large pieces of inoculum and substratum from agar 

 slopes were placed in Erlenmeyer flasks containing the liquid 1-8" deep. Care 

 was taken not to drown the inoculum. Scant surface, but a large amount of 

 sub-surface, growth in the medium was the result. The amoui^t of differen- 

 tiated mycelium bearing the oospore-like bodies was very marked. Time did 

 not allow making careful search for antheridia, but later further studies will be 

 made. 



The average size of these bodies taken from various cultures, solid and 

 liquid, varied around 33u. 



Stained sections of diseased potato leaves which were allowed to rot in 

 moist sand showed many spherical bodies about 6u in diameter. The nature 

 of these bodies is unknown. Attempts at infection of potato tubers and leaves 

 with the rotten material failed. 



Dept. of Botany, 



Macdonald College, P. Q., Canada. 



References. 



1 . Jones, L. R. Resting Spores of the Potato Fungus. Science N. S. 



30: 813-814 1909. 



2. Clinton, G. P. Conn. State Station Rept. 1909-1910: 713-774. 



3. Berlesee A. N. Saggio di una Monagraphia delle Peronosporacea, 



Portici, 1903. 



WINTERING OVER AND INFECTION OF PUCCINIA MALVACEARUM, 



MONT. 



H. Hill, B.S.A. 



This paper consists of a brief report of germination tests and inoculations 

 made with the teliospores of Puccinia Malvacearum which had overwintered 

 outdoors in Quebec on the stems and leaves of the hollyhock. 



The purpose of the work was to determine the possibility of the organism 

 living over the winter as a teliospore and germinating the following spring, 

 causing infection of healthy plants. 

 6 



