Diseases of Fishin the Adirondacks 19 
and failed to pass into solution. It was further treated with concen- 
trated nitric acid when the stained product passed immediately into 
solution. The nitric acid was allowed to evaporate completely. Upon 
a re-examination of the slide some thirty or more well defined minute 
crystals appeared. These were magnilied 84 diameters and carefully 
studied under polarized light. The first six forms represented in the 
above legend were unquestionably present and perfectly formed. The 
seventh was apparently present. The forms are all simple and in the 
monoclinic system of crystallization. The crystals are soluble in water. 
“Commercial hippuric acid is slowly soluble in water, readily soluble 
in concentrated nitric acid, and similar crystals form upon the evapora- 
tion of these solutions. All of the forms represented in the diagram 
have been duplicated with commercial hippuric acid.” 
The study of sections from several animals reveals the fact that 
these bodies exist in the tissues of the intestinal wall and adjacent 
cells as well as in the lumen. When the stain is extracted, there 
frequently remains a deeply staining center. If these bodies were 
crystalline as Osborne holds, the stain would appear on the edge 
or penetrate along the planes of cleavage. This it does not do. 
If it can be shown that these bodies have accumulated during 
the encysted period of the worm, then it will indicate an unusual 
amount of metabolic activity for an animal in a supposedly quiescent 
state. 
Clinostomum marginatum possesses both organs of generation, the 
testes and ovary. In this sense it is to be regarded as an herma- 
phrodite. The question of whether the eggs are fertilized by the 
sperms grown in the same animal no one has attempted to answer 
because the facts are unknown. From figures one may see the position 
of the two testes and that a convoluted (much coiled) tube serves 
to carry the sperms to the exterior. Osborne says that the testes 
are made up of cells, each of which, in some bass worms, is almost 
completely filled by the very large nucleus. This is poor in chromatin 
and has a very large, readily staining nucleolus which indicates the 
inactive stage preceding development of the sperms. In other bass 
specimens are cells showing various phases of sperm transformation. 
In the worms from the heron the testes contain fully developed 
spermatozoa scattered among the active cells. The ovary is a small 
oval sack located between the testes. The oviduct eventually passes 
externally to the anterior testes on the left side and empties into a 
large sack termed the uterine sack. The uterine sack may be greatly 
distended in mature worms or as seen in optical section appear as a 
