214 ANNUAL REPORT SMITHSONIAN INSTITUTION, 1944 
“pin-point” cells, those cells situated between the normal tissue cells 
and just visible under the ordinary microscope, and to observe the 
smaller cells which compose the interior of these pin-point cells. 
When one of these smaller cells is magnified, still smaller cells are seen 
within its structure. And when one of the still smaller cells, in its turn, 
is magnified, it, too, is seen to be composed of smaller cells. Each 
of the 16 times this process of magnification and resolution can be 
repeated, it is demonstrated that there are smaller cells within the 
smaller cells, a fact which amply testifies as to the magnification and 
resolving power obtainable with the universal microscope. 
More than 20,000 laboratory cultures of carcinoma were grown 
and studied over a period of 7 years by Dr. Rife and his assistants 
in what, at the time, appeared to be a fruitless effort to isolate the 
filter-passing form, or virus, which Dr. Rife believed to be present in 
this condition. Then, in 1932, the reactions in ‘growth of bacterial 
cultures to light from the rare gasses was observed, indicating a new 
approach to the problem. Accordingly, blocks of tissue one-half 
centimeter square, taken from an unulcerated breast carcinoma, were 
placed in triple-sterilized K Medium and these cultures incubated at 
37° C. When no results were forthcoming, the culture tubes were 
placed in a circular glass loop filled with argon gas to a pressure of 
14 millimeters, and a current of 5,000 volts applied for 24 hours, after 
which the tubes were placed in a 2-inch water vacuum and incubated 
at 387° C. for 24 hours. Using a specially designed 1.12 dry lens, equal 
in amplitude of magnification to the 2-mm. apochromatic oil-immer- 
sion lens, the cultures were then examined under the universal micro- 
scope, at a magnification of 10,000 diameters, where very much ani- 
mated, purplish-red, filterable forms, measuring less than one-twen- 
tieth of a micron in dimension, were observed. Carried through 14 
transplants from K Medium to K Medium, this B. X. virus remained 
constant; inoculated into 426 Albino rats, tumors “with all the true 
pathology of neoplastic tissue” were developed. Experiments con- 
ducted in the Rife Laboratories have established the fact that these 
characteristic diplococci are found in the blood monocytes in 92 per- 
cent of all cases of neoplastic diseases. It has also been demonstrated 
that the virus of cancer, like the viruses of other diseases, can be easily 
changed from one form to another by means of altering the media upon 
which it is grown. With the first change in media, the B. X. virus 
becomes considerably enlarged although its purplish-red color remains 
unchanged. Observation of the organism with an ordinary microscope 
is made possible by a second alteration of the media. A third change 
is undergone upon asparagus base media where the B. X. virus is 
transformed from its filterable state into cryptomyces pleomorphia 
