THE BACTERIA IN CONTAGIOUS MALADIES. 161 
into the blood of sheep and of rabbits, these cor- 
puscles produced death with the symptoms of 
charbon, and the blood of the animals presented 
numerous bacteria. The appearance of spores in 
the liquid under cultivation, containing bacteria, 
occurs in twenty-four hours at 35° (95° Fah.), in 
three days at 18°; above 45° and below 12° it is 
no longer possible. Once produced these spores 
resist putrefaction, desiccation, and alternation of 
humidity and dryness, during several years. On 
the contrary, the adult form of the Bacillus an- 
thracis dies under the influence of putrefaction 
and of oscillations of temperature. It has not 
seemed to develop itself in the dog, the cat, birds, 
and cold-blooded animals. The immunity which 
these enjoy has recently been the object of a study 
by Pasteur, Joubert, and Chamberlain. Believing 
that it might be attributed to their temperature, 
incompatible with the life of the bacteria, they 
have refrigerated a fowl, and have ascertained that 
it lost this immunity. Besides, by placing. the 
infected animal in an oven at 30° (86° Fah.) they 
have seen the temperature come back rapidly to 
the normal and the symptoms of charbon to re- 
cede. 
The labors, then, of Koch add an additional 
element of probability in favor of the parasitic 
theory. They show us the existence of an organ- 
ism which we would be able to invoke as the cause 
of spontaneous epizootics. 
All these results Cohn of Breslau has obtained 
upon repeating the experiments of his compatriot. 
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