174 PHYSIOLOGY OF THE BACTERIA. 
again found a few hours before the new access. 
They are no longer found when convalescence is 
established. Heidenreich, Weigert, Birsch-Hirsch- 
feld, and Cohn have confirmed these observations. 
Intermittent Fever.—In all the analyses of air 
made in the vicinity of lands where this fever 
prevails, numerous inferior organisms have been 
found. I refer to a previous work for additional 
information,’ and limit myself to pointing out 
the observations of Griffini, who has found in the 
dew of places subject to this fever, Vibrio bacil- 
lus, V. lineola, Bacterium termo, B. catenula, etc.” 
1 Ant. Magnin, Rech. Geol., Bot. et Stat. sur l’Impaludisme dans les 
Dombes, et le Miasme Paludéen. Paris, 1876. 
2 “ Professors Klebs and Tommassi-Crudelli, who have recently spent 
some time in the neighborhood of Rome with the intention of investigat- 
ing the cause of malarial fevers, have published an account of their 
researches. From an abstract of their report, published in a recent 
number of the ‘ Medical Times and Gazette, we learn that the inves- 
tigators followed a very deliberate plan in the performance of their 
pase 5c): 
“Professors Klebs and Tommassi-Crudelli first succeeded in producing 
the symptoms of malarial poisoning in animals by injection of watery 
extracts from the marshy soil. They then proceeded, by the process 
called ‘ fractional cultivation,’ to isolate the active material, that is, the 
true generator of the disease, supposed to be a living organism. Lastly, 
they isolated the organisms by filtration; and, comparing the results ob- 
tained in injections of the filtrate with those produced by the residue 
containing the organisms, they proved that the poison of malaria resides 
in these. The fungi obtained appeared as small rods of 0.002 to 0.007 mil- ° 
limeter in length, growing into long, twisted threads. The fungus is 
markedly aerobiotic. If air is excluded, it dies out. The injection of 
these fungi—true bacilli malarie—into healthy animals always gives 
rise to symptoms of intermittent fever, with enlargement of the spleen, 
ete. Later, Dr. Marchiafava, at Rome, was able to demonstrate spores 
and bacilli in the spleen, the marrow, and the blood of three persons who 
had died of pernicious fever, showing the same characters as those ob- 
served by Klebs and Crudelli. In summarizing the results of their in- 
vestigations, the authors consider the following facts as proved: 1. That 
