306 Chemical examination of the Hop. 
in a second portion of alcohol, twelve grains more of resin 
were obtained, less bitter, but otherwise like the last. 
Exp. 10th.—The lupulin which was the subject of the 
last experiment, after having been thus boiled in water, and 
digested in aleohol, was put into a small retort, and boiled 
in two ounces of ether. While boiling, it was filtered into 
a vessel containing cold water, by which means twelve 
grains of wax were obtained.* : 
Exp. 11th.—Half an ounce of lupulin was boiled suc- 
cessively in water, alcohol and ether. On weighing the in- 
soluble residuum, it was found that five eighths of the whole 
had been taken up by the solvents. 
From the foregoing experiments, all of which were, with 
some variation, frequently repeated, I infer, that the lupu- 
lin contains a very subtle aroma, which is yielded to water 
and to alcohol, and which is rapidly dissipated by a high 
heat; that no essential oil can be detected by distillation in 
any portion of the hop; that the lupulin contains an ex- 
tractive matter, which is soluble only in water ; that it con- 
tains tannin, gallic acid, and a bitter principle, which are 
soluble in water, and in alcohol; that it contains resin, 
which is dissolved by alcohol and by ether, and wax, which 
is soluble only in alkalies and in boiling ether; that it con- 
tains neither mucilage, gum, nor gum-resin ; that the aro- 
sooner by both when they are hot than when they are cold; 
exist in very nearly the following proportions :—~In tv 
chms (or one hundred and twenty grains) of lupulin, 
ere is, 
that of 
