10 



At the time my former article was written it was too early to assert 

 positively that this species was single brooded. This point is now pos- 

 itively determined, as the larvae obtained from eggs deposited by the 

 iraagos in confinement have been kept in the usual way in a breeding 

 cage until now, when the imagos have emerged. Thus I have the his- 

 tory of this species from imago to imago again, and have the original 

 females for comparison. 



Mr. Harrington's difficulty seems to be a kind of reluctance to recog- 

 nize two distinct species of saw-flies attacking the strawberry and a 

 suspicion that it is only one species with variations. If all the imagos 

 collected by him are Harpiphorus niaculatus Nor. it is evident that the 

 venation, size, and coloration of that species are very variable. It is the 

 evidence pointing to the establishing or separation of the strawberry 

 saw-flies into two species that I wish to consider briefly. 



Dr. C. V. Kiley, who has so thoroughly traced the life history of R. 

 maculatiis, gives a description of the larvae from which the imagos were 

 reared, in the Ameriean Entomologist Vol. I, p. 90. From tliis descrip- 

 tion we find that the larvae of H. maculatus may be distinguished from 

 those of M. ignota by having three large black spots on the head. 

 Thus the question to be determined is whether these characters of 

 the larvae are constant, and whether the imagos reared from them are 

 constant. This I hope to do by presenting proofs for the same or re- 

 ferring to the literature on the subject in such a way that those inter- 

 ested may investigate and decide for themselves. 



Through the kindness of Prof. F. M. Webster, of Lafayette, Ind., I 

 have before me the larvae from which he reared imagos of H. maculatus. 

 These larvae bear the three black spots on the head, as described by 

 Kiley. Through the kindness of Prof. S. A. Forbes I have before me 

 the imagos of H. maculatus, and also a part of the lot of larvae from 

 which they were reared. Again, it is found that the larvae bear the 

 characteristic markings of the head already spoken of. Thus, from the 

 writings of those who have traced the life history of H. maculatus from 

 imago to imago, again it appears that the above-noted larval characters 

 are constant, and that the imagos reared from them are also constant. 



My own experience in rearing imagos from larvae with heads of a 

 uniform yellowish brown color has already been stated in a former 

 article. However, a few observations made by Professor Osborn and 

 my brother, Charles W., at Ames, Iowa, this spring, may be of interest 

 as a check on my work of last year. The breeding cage containing the 

 saw-fly larvae which were reared from eggs deposited in confinement was 

 left in charge of Professor Osborn when I left for Champaign last Octo- 

 ber. This spring Professor Osborn sent me imagos of both sexes taken 

 from this cage, and stated that they had emerged April 16, 1890. 

 About the same time my brother made sweepings on the same straw- 

 berry bed where I had collected last season, and he also sent me a num- 

 ber of specimens. 



