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separate vial, and the same mode of procedure was followed with the 

 whole inside of the thorax, this giving' me the entire bee except the 

 head, anterior and middle legs, wings, and chitinous walls of the thorax 

 and abdomen. Besides these a number of the bees were kept intact. The 

 whole series was submitted to the assistant professor of chemistry of 

 the Ohio State University, L. M. Bloomfield, to be tested for arsenic by 

 the Marsh method. Mr. Bloomfield found the weight of material sub- 

 mitted in each case to be as follows : Posterior legs, with pollen attached, 

 0.3498 gram ; contents of abdomens and honey sacs, 0.0990 gram ; ditto 

 thorax, 0.0710 gram. After the usual tests to prove the absence of 

 arsenic in the reagents it was found that no arsenic was associated 

 with the posterior legs or the pollen with which they were loaded, none 

 had been left in the thoracic matter, but the material from the abdomens 

 gave unmistakable proof of the presence of arsenic. The entire bodies 

 of a number of the bees, taken at the same time from the same tree, 

 were then washed with diluted ammonia water, three washings failing 

 to give a trace of arsenic, but the bodies, after being thus treated, and 

 being boiled in water slightly acidulated, gave distinct traces of the 

 poison, thus eliminating any possibility of the poison having been 

 introduced into the abdominal matter at the time of dissection and 

 from the exterior. May 15 a crab apple tree {Cratwgnu) was sprayed 

 with a mixture of the same ratio of Paris green as before, but in this 

 case only the contents of the abdomens were retained. This matter, to 

 the weight of 0.1463 gram, treated as in the preceding, gave unmis- 

 takable proof of the presence of arsenic. 



Just at this stage of my investigations, chance, if such a thing there 

 be, threw in my way still more conclusive proof. A few days prior to 

 my last experiment, probably about May 10, a small apple orchard on 

 the experiment farm was sprayed with Bordeaux mixture, to which had 

 been added Paris green at the rate of 4 ounces to each 50 gallons of the 

 mixture. The bloom had at this time nearly all fallen from the trees 

 the exceptions being an occasional belated cluster. Three colonies of 

 bees, recently brought on to the premises, were located near by, to all 

 appearances in a perfectly healthy condition. A few days after the 

 application of the poisoned Bordeaux mixture one colony suddenly 

 became extinct and a second greatly reduced in numbers, dead bees 

 being abundant about both hives. From these colonies I was able to 

 secure dead bees, and both honey from uncapped cells and dead brood 

 from the hive that had been so mysteriously depopulated. When tested 

 for arsenic by Mr. Bloomfield, precisely as with the other matter, con- 

 tents of abdomens of the dead bees to the amount of 0.2334 gram 

 revealed the i^resence of arsenic ; 3. 7061 grams of honey gave no trace 

 of poison, while 1.8481 grams dead brood showed it to be present, and 

 the entire bodies of the dead bees, thrice washed in ammonia water, as 

 before explained, gave traces of arsenic. In regard to the honey I can 

 only say that it was from uncapped cells, which might and probably 



