22 Contagious Diseases of the Chinch-bug. 
Two of the a pots showed a decided growth of Sporotrichum, 
while one of a and all of b and ¢ did not show any fungus. 
Experiment 24. September 6, experiment to determine the 
effect of temperature on infectious diseases of chinch-bugs. 
Three pots with bugs sent in by express had Sporotrichum 
added, and were put into a refrigerator. Three pots, similarly 
arranged, were put into a Wardian case at room temperature. 
Experiment started 12 to 12:15 p.m. The temperature was 
taken with a Centigrade thermometer. 
September 7—11:15 a. m.: Wardian case, 24.5°; refrigerator, 13°. 
September 7— 2:45 p.m.: Wardian case, 25°; refrigerator, 11°. 
September 7—5:00 p.m.: Wardian case, 24.5°; refrigerator, 10.75”. 
September 8—10:00 a. m.: Wardian case, 24.5°; refrigerator, 11.5”. 
September 9—7:30 a. m.: Wardian case, 23.5°; refrigerator, 12°. 
September 9—5:25 p. m.: Wardian case, 22°; refrigerator, 11.75°. 
September 10—7:35 a. m.: Wardian case, 26°; refrigerator, 12°. 
Bene 10—7:35 a.M.: One pot of each lot was opened; no disease was 
noted. 
September 12—4:25 p.m.: Very hot afternoon; Wardian case, 31°; refrigera- 
tor, 13° to 14°, probably 14°. 
September 13—9:15 a.m.: Wardian case, 28.5°; refrigerator, 16°. 
September 14—10:15 a.m.: Wardian case, 29°; refrigerator, 14°. 
September 16— about 8:30 a.m.: Refrigerator, 16.5°. (Supply of ice small.) 
Pots taken out September 23; few bugs alive. Not much 
trace of disease, and-no noticeable difference between bugs kept 
in refrigerator and those in Wardian case pots. 
Experiment 25. September 30. Bugs taken from millet and 
corn were put into five pots, three containing wet earth, and 
two dry. All were infected with Sporotrichum, the experiment 
being merely to determine the relative resistance to diseases of 
bugs taken at this period. Dirt was not sterilized. Pots were 
put into a close dark place —a refrigerator without ice. 
October 16— Pot A, wet: Five Sporotrichum bugs seen 
with perhaps 40 to 50 still living and apparently healthy bugs. 
Interior of pot moist, and more water, not distilled or sterilized, 
was added. 
October 16— Pot B, dry: Two or three Sporotrichum bugs 
seen. Growth on these not so large as on those in wet pot. 
May have been a few more Sporotrichum-covered bugs in the 
pot, but number proportionately very small. Fifty to 100 live 
bugs roughly estimated. Pot was left dry. 
October 16—Pot C, wet: Three, very probably Sporotrichum, 
bugs seen. If others were present they were very inconspicu- 
