a 
Fifth Annual Report. 25 
things to learn, if possible, whether the field was thoroughly 
inoculated. In answer to this question we found that the lib- 
eral addition of Sporotrichum by different methods to the bugs 
thus isolated in the boxes did not apparently increase their 
mortality. Sporotrichum was very destructive, as well as Em- 
pusa, which was not added. by us, but the bugs apparently did 
not die of Sporotrichum faster in the boxes inoculated than was 
the case in the open field. 
The inference is reasonable that the field was thoroughly in- 
oculated and would not have profited by further treatment. 
Empusa, however, flourished as well in the boxes as in the 
most favorable situations in the open field, and this may ac- 
count for the fact that the bugs were nearly all dead in the 
boxes at the expiration of 20 days, while in the field they were 
still numerous. 
June 15. Open box No. 1 was started. The bottom of the 
box was covered with garden soil to a depth of from one to 
three inches, and the sides, which were eight inches high, were 
thoroughly chalked down to the soil. Four pots of young field 
corn were arranged along the middle of the box, and after the 
soil had been well dampened, the bugs, taken directly from a 
field, were introduced. There was almost a pint of them, 
mostly pupe. A few fungus-covered bugs were scattered near 
and on the pots. The box was placed near a west window so 
as to get three to four hours’ sunlight every day. This was 
done with the intention of making the conditions in the box as 
nearly as possible like those of an ordinary field. 
June 16. Bugs thick on stalks of corn, causing it to wilt. 
Corn renewed ; very few bugs escaping over the chalk. 
June 17. Quite a number of dead bugs (white) were noticed. 
They were scattered in patches on the surface of the soil. This 
appearance of Sporotrichum so soon after the starting of the 
box was doubtless due to the presence of the disease in the field 
from which the bugs for stocking the box had been taken — 
this field having been infected last year and also this spring. 
June 18. Slight increase in number of dead bugs. Corn 
repotted. 
June 19. No perceptible increase of dead bugs. Results in 
the box are much like those in the corn-field from which the 
