Fifth Annual Report. 43 
mained free from Sporotrichum ; while bugs infected and kept 
in similar jars have been found dead with Sporotrichum on 
them. This has been done at different times and in different 
states. 
2. Localities have been reported in which bugs were abun- 
dant, but no Sporotrichum could be found. 
E.— The validity of the arguments ae D depend on the follow- 
ing assumptions : 
1. That the chinch-bugsin the check-jars were, in all respects, 
under conditions favoring the appearance of the fungus if it had 
been present. 
2. That conditions favoring the development of Sporotrichum 
prevailed in the localities mentioned at the time when the 
fungus was sought; so that if it had been present, it would 
have become visible. Further that the search was complete 
enough to have discovered any fungus-covered bugs if they had 
been present. 
Nore.— Different lots of chinch-bugs kept in jars under conditions, so far as 
known, similar for all, have shown very different results as regards the develop- 
ment of Sporotrichum i in different jars. 
F.—Further evidence on the above question may be sons et from 
the following sources : 
1. Localities may be found in which the chinch-bugs prevail 
that are so situated that it is not possible for Sporotrichum to 
have been artificially introduced. 
2. It may be determined whether Sporotrichum continues to 
survive a long series of winters, as it apparently has done in 
the years 1894-’95. 
We suggest the following final considerations : 
A. 
Sporotrichum was well distributed in this state at the begin 
ning of the season of 1895, so that the question of whether or 
not infection was present does not enter into the explanation of 
the varying results observed in fields where bugs were abun- 
dant. 
B. 
It is very "probable that where Sporotrichum and Empusa 
are present serious epidemics may occur if certain conditions 
