56 STATE UNIVEBiSITY EXPERIMENTAL STATION. 



APPENDIX "B. 



DIRECTIONS FOR OBTAINING AND APPLYING THE CHINCH-BUG 



INFECTION. 



In order to keep up our supply of infected bugs, we must require that each 

 person sending for infection send us live bugs from the field. These should 

 be put into a tin box (a baking powder box is excellent for this purpose), 

 without soil, and with a supply of green wheat or corn. The box should 

 have a tight-fitting cover, and no holes need to be made. We have found 

 that the bugs reach us in the best condition when sent in this way. 



Upon receipt of infected bugs from the Station, a shallow box about 24x36 

 x6 inches, with tight joints, should be provided. Sprinkle the whole inside of 

 the box with water and put in enough green wheat or corn to cover the bot- 

 tom. Scatter the infected bugs over the bottom and put in a large quantity 

 of live bugs from the field ; a quart would not be too many for a box of the 

 size above suggested. A smaller box may be used for a smaller quantity of 

 bugs. After the bugs have remained in the box two days, remove half of 

 them, alive and dead, and scatter them over that part of the field where the 

 bugs are thickest; at the same time replenish the infection box with more 

 bugs from the field. Continue to scatter bugs from the infection box over 

 the field at intervals of two days, until it is seen that the bugs are dying 

 rapidly all over the field. Keep the box moist by repeated sprinkling and 

 change the green food as often as it loses its freshness. 



Careful attention to these directions will often insure success where care- 

 less use of the infection would fail. Make daily notes on the appearance of 

 the bugs in the infection box and in the field, and of the weather while the 

 field infection is in progress. Note carefully the chinch-bug conditions in 

 neighboring fields. Keep a list of farmers who get infected bugs from your 

 farm. 



Save a quantity of fungUs-covered and non-fungus-covered dead bugs in a 

 tin box for use the following year. Put the infection box away for future 

 use. 



Should the first lot of infected bugs from the Station seem to fail in their 

 purpose, send without delay for a new supply. 



Do not fail to send a full report of the experiment to the Director of the 

 Station. 



