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March. In the accompanying letter he remarks that they presented 
the same appearance then as in the November and December pre- 
ceding, his attention having first been called to them at that time. 
On the 10th of April, I visited Cuba, and carefully examined the 
damaged wheat fields. At-this time the larve were abundant at 
the bases of the stalks, all apparently full-grown, being of nearly 
uniform siz2; but no other forms were detected. 
Larve continued to occur in the wheat at Normal, Cuba and 
Decatur as late as May 15, but on the 2€d of that month none 
could be found after long continued search in fields which had pre- 
viously been seriously infested by them. 
The worms of the second brood first appeared in our collections 
on the 26th of June, at which time they occurred in stalks of wheat 
and rye just above the upper joint, in fields near Warsaw, in 
western Illinois. By Prof. Riley, they have been found near St. 
Louis as early as the middle of June, and by Mr. Lintner, in New 
York, as late as ‘‘about the Ist of August.” 
Pupa. The pupa was first obtained by us on the 12th of April, 
in some wheat sent from Centralia, Illinois, a few of the larve 
occurring at the same time. On the 380th of April, a puparium 
which had formed from the larve obtained at Cuba was opened 
and found to contain a pupa apparently on the point of emerg- 
ing; but careful search in the field on the 23d of May showed 
that all of this first brood had transformed to the fly at this date. 
By Prof. Riley, the pupal stage of the second brood is said to last 
from twelve to fourteen days; and Prof. Lintner found the insect 
still in this stage about August 1. 
Imago. I transferred from the infested field at Cuba, a large 
number of the plants containing the larve, and placed them in 
breeding cages at the Laboratory on the 10th of April, for the pur- 
pose of rearing the perfect insect. In order to a certain identitica- 
tion of the fly, pieces of stems of wheat containing the larve were 
isolated in small vials, and kept until the latter transformed. The 
first adult emerged in one of these vials on the 4th of May, and 
others continued to appear in the bottles and breeding cages at 
frequent intervals until June 1, on which date a larva or pupa 
obtained May 15 from Cuba, IIl., emerged as an imago. 
The first specimens of the imago collected in the open air were 
obtained by sweeping strawberry fields on the 23d of May, at which 
date careful search of wheat fields previously infested showed no 
larve or pupe, but numerous adults. On the 24th of May, a num- 
ber of adults were found in sweeping wheat at Normal, and also a 
few in meadows containing June grass and timothy. 
Careful sweepings made in a variety of situations on the 15th of 
June, at Normal, yielded none of the adults, neither could they 
be found on the 25th of June at Warsaw, in western Illinois, in 
fields where the larve of the second brood at that time occurred. 
Clover meadows and other situations adjacent to these infested fields, 
were carefully swept for adults, but none were obtained. At Jer- 
seyville and Alton,.it was likewise impossible to find them, although 
evidence of the work of the larve was not uncommon. 
