119 
ve had developed white mycelium; another was characteristically pink 
in color; while the third showed no signs of disease. The dead larvee 
were placed on damp sand, and by July & one had formed spores of 
s0trytis from which successful cultures of tenella were afterwards ob- 
tained. The check lot developed fewer imagos, and contained a dead 
larva and pupa on June 29. hese failed to develop parasitic fungi 
when placed on damp sand, and this experiment was not carried fi ther. 
Perhaps the most successful attempt to kill our common white 
grubs with this Botrytis in our breeding cages began August 26, 1892, 
when nineteen grubs from a corn field were thoroughly covered with 
spores from a culture made in agar-agar in July. The dusted grubs 
were placed in a breeding cage, without food, and moistened from time 
to time with water. September 24 ten of them were dead, and spores 
had formed on four. Within the bodies of the others a mycelial growth 
was discernible, but none appeared on the outer surface. The cage 
was not again disturbed until March 28, 1893, when eight more dead 
erubs were found, only two of them showing fungus spores. The six 
others contained no mycelium, and probably died from other causes. 
Experiments with Jsaria leprosa, Fr., were begun April 19, 1892, 
in accordance with a suggestion made by Dr. Roland Thaxter, from 
whom a culture tube of this fungus was received March 21. his cul- 
ture was repeated on agar by Professor Burrill of the University of Il- 
linois, and April 19 twenty erubs were dusted with spores from this 
second growth, and placed in an earthen vessel which was filled with 
leaf mould and sunken in the earth. Grubs died in this lot April 23, 
25, and 27, most of them becoming firm to the touch and of a dusky 
brownish hue. The record shows the death of thirteen, in all, of this 
experimental lot up to May 20, at which time ants invaded the cage, 
and the experiment was discontinued. Five dead specimens trans- 
ferred to damp sand May 30, simply decayed without visible fungus 
growth. Only two of the lot, in fact, formed a mycelium; and none 
matured spores. 
‘The second lot of larvee, thirty in number, Cyclocephala and Lach- 
nosterna mixed, was dusted May 25, 1892, with spores of sara le- 
prosa, from the above-mentioned culture on agar. a! were placed in 
a breeding cage and left undisturbed until June 29, when five dead 
larve, fifteen living ones, two pupe, and one adult: oe locephala were 
found, the remaining seven having died and disappeared. Two of those 
taken dead from the cage were placed on damp sand June 29, and grew 
the fungus rapidly in the form of stalk-like bundles which bore spores 
abundantly by July 1. Agar cultures made from these spores on the 
8th of July were indistineuishable from the original culture or from 
the fungus growing on the grubs. There was no appearance of a fun- 
gus growth on any of the grubs in the check lot corresponding. 
"The common parasitic fungus Sporolrichum globuliferum, char- 
acteristic of the so-called white muscardine of the chinch bug, was also 
used by us at this time in these white grub experiments, and seemed on 
the whole more efficient than either Botrytis tenella or Tsaria leprosa. 
April 19, 1892, thirty grubs taken from an old corn field were thor- 
oughly covered with spores of Sporotrichum from a culture made on 
corn meal Fade with beef broth. This culture was derived directly from 
