72 Instructions in Zoology and Animal Physiology, 



Their return to life, truly surprizing by its rapidity, was brought about, 

 in my experiments, by immersion in water slightly heated. Almost at 

 the moment when the particles of ice were melted, the limbs and skin 

 recovered their flexibility, and I saw the animals begin to move; at the 

 same instant their eyes, hitherto very much sunk, suddenly became pro- 

 minent, and in a short time, about ten or even eight minutes, they seemed 

 perfectly restored. 



The toads which I caused to freeze too rapidly, whether in water or 

 air, never came again to life ; at the most they made a few faint move- 

 ments, soon followed by death.* 



Instructions in Zoology and Animal Physiology, for the British 

 Antarctic Expedition, -f- 



1. Marine Invertebrata. — The animals which it is desirable 

 to preserve, and which may at first present themselves to the 

 notice of the naturalist in the present expedition, ai'e the 

 floating marine Mollusca and Crustacea, and those which in- 

 liabit the Sargazzo or Gulf-weed. 



With respect to the Mollusca, all the species of the Cepha- 

 lopoda or Cuttle-fish tribe, and all the Pteropoda or lower or- 

 ganized fioating Mollusca, should be preserved. If taken 

 nlive they should be allowed to die gradually in sea-water, by 

 which means they commonly remain in a relaxed state, and 

 display more of their natural outward form. ^\lien dead they 

 should be soaked for a short time in fresh water, and then 

 put into spirit ; or, if transparent, in the saline solution,;]: to 

 prevent decomposition, which otherwise rapidly takes place. 

 To each specimen should be attached a number, stamped on 

 sheet tin, coi-responding to the entry-number in the Catalogue, 

 in which should be noticed the kind of locomotion, or other 

 vital phenomena, and the colour of the living animal, the 

 latter being speedily altered or lost in the preserving liquor. 

 The larger Crustacea will be liable to become putrid in spirit, 



* Voyage en Islande et au Greenland, par M. P. Gaimard, t. i. pp. 349, &c. 

 t Prepared by the President aiid Council of the Royal .Society. 

 J Connuon salt, . . 1 part. 

 Alum, ... 2 parts. 



Boiling water. . . 10 parts. 

 Filter the solution when cold. • 



