Division in the Root of Vicia Faba. 173 
the spores of Saccharomyces, as observed by Wager, is a 
strong argument in favor of the view here put forward. The 
author’s own observations on the ascospores of Tuber @stivum 
confirm this. He has observed, that at first the nuclei 
formed in the ascus are large, and contain a network of 
chromatin, in which a few nucleoli stained with acid anilin 
dyes, are imbedded. The nucleus enlarges, the chromatin 
disappears, whilst the nucleoli increase in size and number, 
and now exhibit an affinity for basic stains. In this process, 
there is probably at first a transference of chromatin into 
nucleoli and afterward a bodily transference of these latter 
into nuclei. 
In all these cases, if we admit the hereditary functions of the 
chromatin, it is scarcely possible to deny it to the nucleoli. 
My own observations were made on the cells in the grow- 
ing apex of the root of the broad bean (Vicia Fada). 
METHODs. 
As fixatives chromic and chrome-acetic acids were employed, 
both of which gave excellent results. These solutions are 
washed out with water till all the yellow color has disap- 
peared. The material is then gradually passed through alco- 
hols of increasing strength into pure alcohol. As a clear- 
ing fluid, oil of cedar was used, as it is less liable to cause 
shrinkage, than does xylol. First the material was put into 
a mixture of the oil and pure alcohol, next into pure oil, 
thereafter into paraffin, several changes in this last being made 
to insure complete elimination of the oil. All changes from 
one solution to another must be made gradually, as for 
example, from the alcohol to the oil. Sections were cut on 
the microtome with a feed of six w. All staining was done 
on the slide; the best stain employed, and the one from 
which all sketches have been made was iron hematoxylin 
and Bordeaux red. 
