246 Phillips on a Comparative Study of the 
the same cell, though either might be absent, and in portions 
of the trichomes where the cells were actively dividing, he 
observed at times a regular change from cells having gran- 
ules to others destitute of them,and again back to the former 
condition. No chromatophore was found, that which had 
been described as such by other authors being a crystalloid. 
Nuclei could not be demonstrated, hence he denied the 
existence of indirect division. A central body, staining with 
hematoxylin, was found. Vacuoles were not present in 
the normal plants, and their contents when found in path- 
ological material, were not known to him. Division of the 
cell was direct and proceeded very rapidly, often the “col- 
lar-like”’ ingrowth from the cell wall which caused division 
by strangulation formed for the granddaughter cells before 
the daughter cells were fully separated. He found the 
end cells protected by a calyptra, formed by the last cell 
ceasing to divide and forming around itself a hard coat. 
In the mature state, this calyptra was quite diagnostic for 
the species. Another striking feature of the end cells of 
certain Oscillarieee was the presence of what appeared to 
be long sluggish flagella. These, however, he dismissed as 
not characteristic, and as being really parasitic growths, like 
bacteria, having no motion of their own. They did show 
a certain movement, he admitted, but he referred it to the 
oscillation of the trichome. 
In 1888, Scott (71) exhibited some slides and reported 
the work of Miss H. V. Klaassen before the Linnean Soci- 
ety. The work was performed upon Tolypothrix cooctitis 
and three species of Oscillaria. He used two methods: (a) 
by treatment for five minutes in methylated ether, and then 
staining for four minutes in Kleinenberg’s hematoxylin and 
mounting in balsam, or (6) by staining for two hours in 
picro-nigrosin and clearing for two minutes in chloral 
hydrate and mounting in pure glycerin. By either of these 
methods he recorded a rounded central body composed of 
fibrils, very similar to the skein stage of the karyokinetically 
