252 Phillips on a Comparative Study of the 
tral body” or nucleus. Fischer acknowledged that the “red 
granules” described by Biitschli were present, but that they 
had been known for a long time and could be seen in the 
_ living cells, though he did not consider them to be of any 
morphological value. Fischer, however, failed to tell where 
they were recorded prior to Butschli’s description, and in as 
extended a search as could be made in the present investiga- 
tion, it has been impossible to locate any reference to them 
of an earlier date. 
To these objections of Fischer, Butschli (8) observed that 
some reagents did produce plasmolysis, but this was not the 
usual case, and when properly handled, it did not occur at 
all with the reagents used by him, which were Flemming’s 
chrom-osmic acid mixture, picro-sulphuric acid, with and 
without having had osmic acid added to it, and osmic 
acid alone. He fortified himself behind the fact that most 
of the recent investigators had observed the colorless “‘cen- 
tral body” in the Cyanophycez, and concluded that Fischer’s 
criticism was based upon unfinished and hasty observations. 
In another work (27) based upon much more extended 
studies of the structure of Bacteria, Fischer reaffirmed his 
former position, and objected still more strongly to the 
methods and conclusions of Biitschli, who thereupon pub- 
lished the results of two very carefully performed investiga- 
tions (9, 10) in which he reaffirmed his former views, 
enlarged and fortified his position, and was able to demon- 
strate the comb-like structure in the “central body” of the 
living cell, though he pointed out differences in one and the 
same species. Beyond this he added very little to his former 
papers except to refute the conception of the “red granules” 
as held by Kunster (44) and Busquet. 
Hieronymus (41) studied the Phycochromacez and con- 
cluded that a nucleus was present. It was composed of 
granules upon a thread very similar to the spirem stage 
of division in higher plants. These nuclear granules con- 
tained a substance which represented, and performed all of 
