io6 Transactions British Mycological Society. 



{a) is still discernible in the middle of the growth on the right, 

 while on the left the young conidiophore borne on the hypha 

 {h, h) is shown. Although this hypha is drawn in the figure 

 with partially discontinuous walls, it was, as a matter of fact, 

 continuous. The figure was drawn with the aid of a camera 

 lucida which did not permit of the whole growth being outlined 

 in one single field of view. 



A stock pure culture was raised from a single ascospore, and 

 this was made the basis of a series of sub-cultures on seven 

 different media. A stock pure culture of V. cinnaharinum 

 was raised from a single conidium of the fungus growing on a 

 decayed potato, and sub-cultures from it were made on the 

 same media. No differences, either macro- or micro-scopic, 

 were discernible in the growths developed in the series of parallel 

 cultures. Hence it is concluded that the perithecia, originally 

 found on the surfaces of the rotting tubers in very intimate 

 association with the conidial stage of V. cinnaharinum, are 

 indeed the perfect state of fructification of this fungus*. This 

 species, therefore, must be removed from the Fungi Imperfecti 

 and be placed amongst the Ascomycetes. 



The ripe perithecia are spherical or globular in shape and 

 possess an ostiole, but are scarcely papillate. They are 

 " cameo-brown "f in colour and bear short, stiff multi-cellular 

 hairs or appendages on their upper halves. A stroma is 

 present which in some cases bears only one perithecium, and 

 in others more than one. The wall of the perithecium is 

 several cells in thickness and more or less leathery, or carti- 

 laginous in substance, not brittle. Long paraphyses are 

 present in young perithecia, at any rate, but they are not 

 easily made out and they disappear later on. They are more 

 easily seen in the carefully teased out contents of a perithecium 

 which is not too ripe than in sections. The asci are typically 

 eight-spored but a lesser number sometimes occurs. The 

 ascospores are arranged in one row and are i-septate when 

 ripe, single-celled when young. The walls of the paraphyses 

 and asci appear to become mucilaginous, so that a fully ripe 

 perithecium contains a mass of isolated ascospores embedded 

 in a more or less gelatinous matrix. Fig. 4, Plate III, represents 

 a longitudinal section (not quite a median one and slightly 

 diagrammatic) through a perithecium and its stroma. The asci 



* After this pure culture work was completed a case was met with in which 

 one of the actual appendages of a perithecium had developed a conidiophore 

 of V. cinnaharinum as a lateral branch, proceeding from near its distal end. 

 This affords yet another link in the chain of proof that the fungus producing 

 the perithecia is V. cinnaharinum. 



t Ridgway, R. Color Standards and Color Nomenclature. Washington, 

 D. C, 1912. Plate 28. 7" k. 



