﻿ot 
  

  

  have 
  caused 
  their 
  death. 
  June 
  27, 
  ten 
  specimens 
  removed; 
  July 
  2, 
  

   no 
  appearance 
  of 
  muscardine. 
  June 
  29, 
  forty 
  dead 
  specimens 
  put 
  

   on 
  moist 
  sand; 
  July 
  2, 
  no 
  evidence 
  of 
  muscardine 
  or 
  other 
  mold. 
  

   June 
  'dO, 
  thirty 
  specimens 
  put 
  on 
  moist 
  sand; 
  July 
  2, 
  no 
  muscardine 
  

   or 
  other 
  mold. 
  June 
  30, 
  another 
  lot 
  of 
  twenty-five 
  specimens 
  placed 
  

   on 
  moist 
  sand; 
  July 
  2. 
  one 
  imbedded 
  in 
  a 
  growth 
  of 
  Entomo}Mhora 
  

   aphidis: 
  no 
  Sporotrichum. 
  July 
  2, 
  no 
  appearance 
  of 
  muscardine. 
  

   Total, 
  105 
  dead 
  examined; 
  one 
  example 
  of 
  Entomophthora, 
  none 
  of 
  

   Sporotri(-hum. 
  

  

  July 
  8. 
  Examined 
  by 
  dissection 
  a 
  dead 
  pupa 
  from 
  the 
  above 
  lot 
  

   while 
  plump 
  and 
  fresh, 
  with 
  no 
  appearance 
  of 
  decomposition 
  or 
  post 
  - 
  

   mortem 
  change 
  of 
  any 
  kind. 
  No 
  trace 
  of 
  fungous 
  affection 
  found. 
  

   The 
  cellular 
  structure 
  of 
  the 
  intestinal 
  cceca 
  had, 
  however, 
  disap- 
  

   peared, 
  and 
  these 
  organs 
  were 
  full 
  of 
  fat 
  globules 
  of 
  different 
  sizes, 
  

   evidently 
  originating 
  in 
  the 
  disorganized 
  contents 
  of 
  the 
  epithelial 
  

   cells, 
  and 
  were 
  also 
  crammed 
  with 
  the 
  usual 
  BaciUus 
  msectoruhi 
  of 
  

   the 
  chinch-bug. 
  No 
  nuclei, 
  cell 
  walls, 
  or 
  definite 
  structures 
  in 
  

   these 
  cd^ca. 
  The 
  cellular 
  structure 
  was 
  also 
  obscure 
  in 
  other 
  

   parts 
  of 
  the 
  food 
  canal, 
  but 
  there 
  was 
  no 
  fatty 
  degeneration 
  there 
  

   and 
  there 
  were 
  no 
  bacteria. 
  If 
  the 
  cteca 
  were 
  crushed, 
  clouds 
  of 
  

   bacteria 
  escaped, 
  luit 
  if 
  other 
  portions 
  of 
  the 
  alimentary 
  canal 
  were 
  

   similarly 
  crushed, 
  only 
  spherical 
  granules 
  of 
  various 
  sizes 
  appeared 
  

   in 
  the 
  fluids. 
  Cause 
  of 
  death 
  obscure, 
  but 
  presumptive 
  evidence 
  of 
  

   injurious 
  action 
  of 
  c(ecal 
  bacteria, 
  causing 
  degeneration 
  and 
  disor- 
  

   ganization 
  of 
  coecal 
  epithelium 
  as 
  at 
  least 
  a 
  feature 
  of 
  the 
  condition. 
  

  

  July 
  27. 
  1896. 
  T 
  dissected 
  two 
  young 
  chinch-bugs, 
  hatched 
  from 
  the 
  

   egg 
  within 
  two 
  or 
  three 
  days 
  previous 
  in 
  my 
  laboratory, 
  for 
  the 
  pur- 
  

   pose 
  of 
  determining 
  the 
  condition 
  of 
  the 
  alimentary 
  cei^ca 
  at 
  the 
  time, 
  

   and 
  especially 
  to 
  ascertain 
  whether 
  these 
  organs 
  contained 
  bacteria 
  

   from 
  the 
  egg. 
  These 
  young 
  bugs 
  had 
  hatched 
  from 
  eggs 
  enclosed 
  in 
  

   a 
  Petri 
  dish, 
  in 
  which 
  they 
  had 
  since 
  been 
  kept, 
  and 
  they 
  conse- 
  

   quently 
  had 
  no 
  opportunity 
  to 
  feed. 
  I 
  found 
  the 
  ca^ca 
  developed 
  in 
  

   both, 
  though 
  most 
  satisfactorily 
  shown, 
  by 
  the 
  dissection, 
  in 
  one 
  of 
  

   them, 
  where 
  they 
  were 
  well 
  and 
  completely 
  displayed. 
  In 
  this 
  there 
  

   •were 
  ten 
  c(eca, 
  two 
  of 
  which 
  were 
  much 
  longer 
  than 
  the 
  others. 
  The 
  

   eight 
  shorter 
  ones 
  were 
  scarcely 
  longer 
  than 
  broad, 
  and 
  consisted 
  of 
  an 
  

   external 
  basement 
  membrane 
  of 
  flattened 
  cells 
  with 
  small 
  flat 
  nuclei, 
  

   and 
  within 
  this 
  a 
  single 
  series 
  of 
  large 
  spherical 
  cells 
  with 
  no 
  inter- 
  

   cellular 
  spaces 
  except 
  a 
  sufficiently 
  conspicuous 
  lumen. 
  They 
  were 
  

   given 
  off' 
  as 
  blunt, 
  broad 
  appendages 
  from 
  a 
  somewhat 
  enlarged 
  por- 
  

   tion 
  of 
  the 
  alimentary 
  canal, 
  in 
  front 
  of 
  which 
  were 
  two 
  distinct 
  en- 
  

   largements, 
  one 
  corresponding 
  evidently 
  to 
  the 
  anterior 
  stomac.i 
  of 
  

   the 
  adult 
  bug, 
  and 
  the 
  other 
  representing 
  apparently 
  both 
  the 
  second 
  

   and 
  third 
  stomachs. 
  There 
  were 
  no 
  bacteria 
  in 
  any 
  of 
  these 
  append- 
  

   ages, 
  or 
  in 
  any 
  other 
  part 
  of 
  either 
  of 
  these 
  bugs. 
  Careful 
  study 
  was 
  

   made 
  of 
  the 
  contents 
  of 
  the 
  organs 
  while 
  still 
  entire, 
  and 
  of 
  the 
  flviids 
  

   escaping 
  from 
  them 
  after 
  crushing 
  them 
  in 
  water. 
  It 
  consequently 
  

  

  