﻿83 
  

  

  them. 
  Ground 
  rather 
  damp 
  from 
  rains 
  within 
  last 
  twenty-four 
  

   hours. 
  April 
  23, 
  2 
  p. 
  m., 
  surface 
  temperature 
  of 
  ground 
  68.5° 
  Fahr. 
  

   Slight 
  development 
  of 
  impure 
  fungus 
  growth 
  on 
  corn-meal 
  — 
  some 
  

   of 
  it 
  apparently 
  Sporotrichum 
  : 
  none 
  on 
  agar. 
  No 
  dead 
  chinch- 
  

   bugs; 
  living 
  beginning 
  to 
  desert 
  their 
  winter 
  quarters. 
  April 
  24, 
  

   surface 
  temperature 
  74° 
  at 
  2: 
  30 
  p. 
  m.; 
  ground 
  rather 
  dry. 
  Neither 
  

   living 
  or 
  dead 
  chinch-bugs 
  in 
  the 
  grass. 
  Traces 
  of 
  original 
  material 
  

   distributed 
  have 
  all 
  disappeared. 
  Agar 
  culture 
  without 
  growth. 
  

   April 
  25. 
  surface 
  temx)erature 
  at 
  3 
  p. 
  m. 
  72°. 
  Growth 
  on 
  corn-meal 
  

   medium 
  more 
  abundant 
  on 
  under 
  side 
  of 
  mass, 
  where 
  it 
  has 
  shrunken 
  

   away. 
  from 
  glass; 
  this 
  part 
  unquestionably 
  Sporotrichum. 
  Beginning 
  

   growth 
  of 
  Sporotrichum 
  in 
  agar 
  tube. 
  A 
  few 
  remaining 
  chinch- 
  

   bugs 
  found 
  in 
  grass, 
  but 
  no 
  dead 
  and 
  no 
  trace 
  of 
  fungous 
  disease. 
  

   April 
  29, 
  surface 
  temperature 
  71° 
  at 
  2: 
  30 
  p. 
  m. 
  Very 
  good 
  growth 
  

   of 
  Sporotrichum 
  on 
  surface 
  of 
  agar 
  and 
  well-marked 
  in 
  fruit-jars. 
  

   By 
  May 
  6 
  the 
  corn-meal 
  culture 
  of 
  this 
  muscardine 
  fungus 
  was 
  pro- 
  

   fuse 
  and 
  covered 
  the 
  entire 
  surface; 
  but 
  that 
  on 
  agar 
  was 
  less 
  

   abundant. 
  

  

  Temperature 
  Ex])eriments 
  on 
  Chinch-bugs. 
  — 
  For 
  the 
  purpose 
  

   of 
  determining 
  the 
  capacity 
  of 
  the 
  chinch-bug 
  to 
  endure 
  heat, 
  a 
  large 
  

   number 
  of 
  experiments 
  were 
  tried 
  in 
  my 
  laboratory 
  with 
  chinch-bugs 
  

   of 
  various 
  ages 
  exposed 
  to 
  temperatures 
  ranging 
  from 
  100° 
  to 
  139° 
  

   Fahr., 
  for 
  periods 
  varying 
  from 
  half 
  an 
  hour 
  to 
  thirty-six 
  hours, 
  under 
  

   conditions 
  to 
  compare 
  the 
  effects 
  of 
  dry 
  and 
  moist 
  heat 
  res^Dectively 
  at 
  

   the 
  various 
  temperatures 
  tested. 
  The 
  apparatus 
  used 
  for' 
  this 
  pur- 
  

   pose 
  was 
  an 
  ordinary 
  bacteriological 
  sterilizer 
  (Arnold 
  Steam 
  Steril- 
  

   izer) 
  with 
  an 
  automatic 
  temperature 
  regulator. 
  The 
  insects 
  ex- 
  

   posed 
  were 
  commonly 
  placed 
  in 
  glass 
  Petri 
  dishes, 
  with 
  paper 
  in 
  the 
  

   bottom, 
  — 
  either 
  moist 
  or 
  dry, 
  — 
  or, 
  in 
  some 
  cases, 
  in 
  moist 
  earth. 
  In 
  

   experiments 
  to 
  determine 
  the 
  effect 
  of 
  moist 
  heat 
  a 
  portion 
  of 
  a 
  

   green 
  corn 
  leaf 
  was 
  commonly 
  enclosed 
  with 
  the 
  insects 
  as 
  food. 
  

  

  The 
  particulars 
  and 
  results 
  of 
  these 
  experiments 
  are 
  best 
  exhibited 
  

   in 
  tabular 
  form. 
  

  

  